Overview

  • Product name

    Human Factor D ELISA Kit
    See all Factor D kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 4 pg/ml
  • Recovery

    < 130 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 126.4 115% - 133%
    Serum 130.3 118% - 139%
    Plasma 131.1 107% - 140%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Human Factor D ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Factor D in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Factor D coated on a 96-well plate. Standards and samples are pipetted into the wells and Factor D present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Factor D antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Factor D bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-human Adipsin 2 vials
    Factor D Microplate (12 strips x 8 wells) 1 unit
    Recombinant human Fatcor D Standards (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
  • Involvement in disease

    Complement factor D deficiency (CFDD) [MIM:613912]: An immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Adipsin
    • ADN
    • C3 convertase activator
    • CFAD_HUMAN
    • CFD
    • Complement factor D
    • Complement factor D preproprotein
    • D component of complement
    • DF
    • PFD
    • Properdin factor D
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab99969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab99969.

  • Representative Standard Curve using ab99969.

Protocols

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.
Protease inhibitors are not necessary for testing serum or plasma samples. We only recommend using inhibitors when testing lysate samples.
Adding protease inhibitors into serum / plasma will not interfere with the assay though.
I hope this helps and if I can assist further, please do not hesitate to contact me.

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