Human Factor IX ELISA Kit (ab108831)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Human Factor IX ELISA Kit
See all Factor IX kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 2.5% Inter-assay Sample n Mean SD CV% Overall 4.6% -
Sample type
Cell culture supernatant, Milk, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.4 ng/ml -
Range
1.56 ng/ml - 100 ng/ml -
Recovery
99 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Factor IX Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor IX concentrations in cell culture supernatants, serum and plasma.
A Factor IX specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor IX specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor IX captured in plate.
Get higher sensitivity in only 90 minutes with Human Factor IX ELISA Kit (ab188393) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Factor IX Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Factor IX Microplate (12 x 8 well strips) 1 unit Factor IX Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Relevance
Factor IX is a vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. -
Cellular localization
Secreted -
Alternative names
- Cf-9
- Cf9
- Christmas factor
see all -
Database links
- Entrez Gene: 2158 Human
- Omim: 300746 Human
- SwissProt: P00740 Human
Associated products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab108831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Sandwich ELISA - Factor IX Human ELISA Kit (ab108831)Human Factor IX standard curve: mean of duplicates (+/- SD) -
Sandwich ELISA - Factor IX Human ELISA Kit (ab108831)Human Factor IX measured in biological fluids showing quantity (ng) per mL of tested sample. Human serum and plasma were diluted 400-3200 fold. Human milk was diluted 1-8 fold.
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Typical Standard CurveRepresentative Standard Curve using ab108831
Protocols
References
This product has been referenced in:
- Liang Y et al. Coagulation cascade and complement system in systemic lupus erythematosus. Oncotarget 9:14862-14881 (2018). Read more (PubMed: 29599912) »
