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Human Factor IX ELISA Kit (ab108831)

Submit a review Q&A (2)References (2)
Sandwich ELISA - Factor IX Human ELISA Kit (ab108831)
  • Sandwich ELISA - Factor IX Human ELISA Kit (ab108831)
  • Typical Standard Curve

Key features and details

  • Sensitivity: 0.58 ng/ml
  • Sample type: Cell culture supernatant, Milk, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Factor IX ELISA Kit
    See all Factor IX/PTC kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5.2%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.9%

  • Sample type

    Cell culture supernatant, Milk, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    = 0.58 ng/ml

  • Range

    0.313 mIU/ml - 20 mIU/ml

  • Assay time

    4h 00m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Factor IX Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor IX concentrations in cell culture supernatants, serum and plasma.


    A Factor IX specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor IX specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor IX captured in plate.


    Get higher sensitivity in only 90 minutes with Human Factor IX ELISA Kit (ab188393) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    70X Biotinylated Human Factor IX Antibody 1 x 90µl
    Chromogen Substrate 1 x 7ml
    Factor IX Microplate (12 x 8 well strips) 1 unit
    Factor IX Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 11ml

  • Research areas

  • Function

    Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.

  • Tissue specificity

    Synthesized primarily in the liver and secreted in plasma.

  • Involvement in disease

    Defects in F9 are the cause of recessive X-linked hemophilia B (HEMB) [MIM:306900]; also known as Christmas disease.
    Note=Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide.
    Defects in F9 are the cause of thrombophilia due to factor IX defect (THR-FIX) [MIM:300807]. A hemostatic disorder characterized by a tendency to thrombosis.

  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.

  • Domain

    Calcium binds to the gamma-carboxyglutamic acid (Gla) residues and, with stronger affinity, to another site, beyond the Gla domain.

  • Post-translational
    modifications

    Activated by factor XIa, which excises the activation peptide.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Christmas Disease
    • Christmas factor
    • Coagulant factor IX
    • Coagulation factor 9
    • Coagulation factor IX
    • Coagulation factor IXa heavy chain
    • F9
    • FA9_HUMAN
    • Factor 9
    • Factor IX Deficiency
    • FactorIX
    • FIX
    • Haemophilia B
    • HEMB
    • MGC129641
    • MGC129642
    • P19
    • Plasma Thromboplastic Component
    • Plasma thromboplastin component
    • PTC
    see all

  • Database links

    Associated products

    Images

    • Sandwich ELISA - Factor IX Human ELISA Kit (ab108831)
      Sandwich ELISA - Factor IX Human ELISA Kit (ab108831)
      Human Factor IX standard curve: mean of duplicates (+/- SD)
    • Sandwich ELISA - Factor IX Human ELISA Kit (ab108831)
      Sandwich ELISA - Factor IX Human ELISA Kit (ab108831)

      Human Factor IX measured in biological fluids showing quantity (ng) per mL of tested sample. Human serum and plasma were diluted 400-3200 fold. Human milk was diluted 1-8 fold.  

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108831

    References (2)

    Publishing research using ab108831? Please let us know so that we can cite the reference in this datasheet.

    ab108831 has been referenced in 2 publications.

    • Cohen CT  et al. Production and control of coagulation proteins for factor X activation in human endothelial cells and fibroblasts. Sci Rep 10:2005 (2020). PubMed: 32029851
    • Liang Y  et al. Coagulation cascade and complement system in systemic lupus erythematosus. Oncotarget 9:14862-14881 (2018). PubMed: 29599912

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Question

    What is the activity of the provided standards?

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    Answer

    Thank you for your call yesterday, and I apologize again for the delay in gettingthis information to you.

    I have contacted the production lab again, and they haverecentlydone further testing to determine that the 400 ng standard isequal to 80 mU. So, the 100 ng/mL standard solution is 20 mU/mL, etc. This information wasgleaned bycomparing ultra pure protein and WHO reference standard, and the assay has been calibrated against many parameters.

    I hope this information will be useful, but if there is anything else that we can do for you please let me know and I'll be happy to help.

    Read More

    Question

    I want to use this kit for xenograft studies.  Does this kit cross-react with mouse Factor IX?

    Read More
    Answer

    Thank you for contacting us.  Based on the lab's data, this kit does not react with mouse Factor IX.  I would still recommend doing a negative control with a WT mouse sample to ensure that your data is accurate and that you are only measuring human Factor IX.  I hope this helps, please let me know if you need any additional information.

    Read More

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