Overview

  • Product name

    Human Factor IX ELISA Kit (with plasma controls)
    See all Factor IX kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall = 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall = 7.1%
  • Sample type

    Cell culture supernatant, Serum, Plasma, Cerebral Spinal Fluid
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    > 1.5 ng/ml
  • Range

    1.56 ng/ml - 100 ng/ml
  • Recovery

    = 97 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Factor IX Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor IX levels in cell culture supernatants, serum and plasma.


    A Factor IX specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor IX specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor IX captured in plate.


    Get higher sensitivity in only 90 minutes with Human Factor IX ELISA Kit (ab188393) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Factor IX Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Factor IX Microplate (12 x 8 well strips) 1 unit
    Factor IX Standard 1 vial
    Negative control (Depleted Human Plasma) 1 vial
    Positive control (Reference Plasma Control) 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Relevance

    Factor IX is a vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease.
  • Cellular localization

    Secreted
  • Alternative names

    • Cf-9
    • Cf9
    • Christmas factor
    • Coagulation factor IX
    • F9 p22
    • Factor 9
    • Factor IX F9
    • FIX
    • FIX F9
    • HEMB
    • P19
    • Plasma thromboplastic component
    • Plasma Thromboplastin Component
    • PTC
    • THPH8
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab168546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab168546

Protocols

References

ab168546 has not yet been referenced specifically in any publications.

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