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    human-factor-v-elisa-kit-ab137976.pdf

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Cardiovascular Blood Serum Proteins
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Human Factor V ELISA Kit (ab137976)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Standard Curve

    Key features and details

    • Sensitivity: 72 pg/ml
    • Range: 0.117 ng/ml - 30 ng/ml
    • Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Overview

    • Product name

      Human Factor V ELISA Kit
      See all Factor V kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall 4.9%
      Inter-assay
      Sample n Mean SD CV%
      Overall 7.2%
    • Sample type

      Cell culture supernatant, Milk, Urine, Plasma, Cerebral Spinal Fluid
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      > 72 pg/ml
    • Range

      0.117 ng/ml - 30 ng/ml
    • Recovery

      97 %

      Sample specific recovery
      Sample type Average % Range
      Milk 91% - 103%
      Urine 94% - 102%
      Plasma 94% - 104%
    • Assay time

      4h 00m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s Factor V Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor V in plasma, urine, milk, and cell culture supernatants.


      A Factor V specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor V specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor V captured in plate.


      Factor V Standard is a native protein.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      100X Streptavidin-Peroxidase Conjugate 1 x 80µl
      10X Diluent M Concentrate 1 x 30ml
      20X Wash Buffer Concentrate 2 x 30ml
      50X Biotinylated Human Factor V Antibody 1 x 120µl
      Chromogen Substrate 1 x 7ml
      Factor V Microplate (12 x 8 well strips) 1 unit
      Factor V Standard 1 vial
      Sealing Tapes 3 units
      Stop Solution 1 x 11ml
    • Research areas

      • Cardiovascular
      • Blood
      • Serum Proteins
      • Cardiovascular
      • Blood
      • Coagulation
      • Common
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Complement ELISA kits
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Blood coagulation ELISA kits
    • Function

      Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
    • Tissue specificity

      Plasma.
    • Involvement in disease

      Factor V deficiency
      Thrombophilia due to activated protein C resistance
      Budd-Chiari syndrome
      Ischemic stroke
      Pregnancy loss, recurrent, 1
    • Sequence similarities

      Belongs to the multicopper oxidase family.
      Contains 3 F5/8 type A domains.
      Contains 2 F5/8 type C domains.
      Contains 6 plastocyanin-like domains.
    • Domain

      Domain B contains 35 x 9 AA tandem repeats, and 2 x 17 AA repeats.
    • Post-translational
      modifications

      Thrombin activates factor V proteolytically to the active cofactor, factor Va (formation of a heavy chain at the N-terminus and a light chain at the C-terminus).
      Sulfation is required for efficient thrombin cleavage and activation and for full procoagulant activity.
      Activated protein C inactivates factor V and factor Va by proteolytic degradation.
      Phosphorylation sites are present in the extracellular medium.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P12259 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Activated protein C cofactor
      • APC cofactor
      • Coagulation factor V
      • coagulation factor V (proaccelerin, labile factor)
      • coagulation factor V jinjiang A2 domain
      • Coagulation factor V light chain
      • F5
      • FA5_HUMAN
      • Factor V Leiden
      • FactorV
      • FVL
      • Labile factor
      • PCCF
      • Proaccelerin
      • proaccelerin, labile factor
      • Protein C cofactor
      • RPRGL1
      • THPH2
      see all
    • Database links

      • Entrez Gene: 2153 Human
      • Omim: 612309 Human
      • SwissProt: P12259 Human
      • Unigene: 30054 Human

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab137976

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab137976? Please let us know so that we can cite the reference in this datasheet.

      ab137976 has not yet been referenced specifically in any publications.

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