Key features and details
- Sensitivity: 6 ng/ml
- Range: 6.25 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Plasma, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Factor VII ELISA Kit
See all Factor VII kits
Intra-assay Sample n Mean SD CV% Overall 4.9% Inter-assay Sample n Mean SD CV% Overall 8.6%
Sample typeCell culture supernatant, Saliva, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity= 6 ng/ml
Range6.25 ng/ml - 200 ng/ml
Assay time4h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Factor VII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor VII concentrations in Human cell culture supernatants, serum and plasma.
A Factor VII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor VII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor VII captured in plate. This assay recognizes FVII and FVIIa.
Get higher sensitivity in only 90 minutes with Human Factor VII ELISA Kit (ab190810) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Factor VII Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Factor VII Microplate (12 x 8 well strips) 1 unit Factor VII Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml
FunctionInitiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
Involvement in diseaseDefects in F7 are the cause of factor VII deficiency (FA7D) [MIM:227500]. FA7D is a rare hereditary hemorrhagic disease. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Numerous subjects are completely asymptomatic despite a very low F7 level.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.
modificationsThe vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
- Information by UniProt
- Coagulation factor VII
- coagulation factor VII (serum prothrombin conversion accelerator)
- Eptacog alfa
SimpleStep ELISA kits
ab108829 has been referenced in 3 publications.
- Liang Y et al. Coagulation cascade and complement system in systemic lupus erythematosus. Oncotarget 9:14862-14881 (2018). PubMed: 29599912
- Mashayekhi A et al. Functional and Molecular Characterization of C91S Mutation in the Second Epidermal Growth Factor-Like Domain of Factor VII. Iran J Biotechnol 16:e1813 (2018). PubMed: 30555841
- Mashayekhi A et al. In vitro expression of mutant factor VII proteins and characterization of their clinical significance. Mol Med Rep 17:2738-2742 (2018). PubMed: 29207137