Key features and details
- Sensitivity: 0.15 ng/ml
- Range: 2 ng/ml - 22.5 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Factor VII ELISA Kit (with plasma controls)
See all Factor VII kits
Intra-assay Sample n Mean SD CV% Overall 2.9% Inter-assay Sample n Mean SD CV% Overall 8.4%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity> 0.15 ng/ml
Range2 ng/ml - 22.5 ng/ml
Assay time4h 00m
Species reactivityReacts with: Human
Abcam’s Factor VII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor VII levels in cell culture supernatants, serum and plasma.
A Factor VII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor VII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor VII captured in plate.
Get results in 90 minutes with Human Factor VII ELISA Kit (ab190810) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Factor VII Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Factor VII Microplate (12 x 8 well strips) 1 unit Factor VII Standard 1 vial Low control (Depleted Human Plasma) 1 vial Positive control (Reference Plasma Control) 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml
FunctionInitiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
Involvement in diseaseFactor VII deficiency
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.
modificationsThe vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
O- and N-glycosylated. N-glycosylation at Asn-205 occurs cotranslationally and is mediated by STT3A-containing complexes, while glycosylation at Asn-382 is post-translational and is mediated STT3B-containing complexes before folding. O-fucosylated by POFUT1 on a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines.
- Information by UniProt
- Coagulation factor VII
- Coagulation factor VII (serum prothrombin conversion accelerator)
SimpleStep ELISA kits
ab168545 has been referenced in 2 publications.
- Cohen CT et al. Production and control of coagulation proteins for factor X activation in human endothelial cells and fibroblasts. Sci Rep 10:2005 (2020). PubMed: 32029851
- Wang H et al. N-Glycan-calnexin interactions in human factor VII secretion and deficiency. Int J Biochem Cell Biol 113:67-74 (2019). PubMed: 31185295