Human Factor X ELISA Kit (ab108832)

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Overview

  • Product name

    Human Factor X ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%

  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    = 1.5 ng/ml

  • Range

    1.563 ng/ml - 100 ng/ml

  • Recovery

    97.5 %

  • Assay time

    4h 00m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s Factor X Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor X concentrations in plasma, serum, milk, urine, saliva and cell culture supernatants.


    A Factor X specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor X specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor X captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Factor X Microplate (12 x 8 well strips) 1 unit
    Factor X Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml

  • Research areas

  • Function

    Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.

  • Tissue specificity

    Plasma; synthesized in the liver.

  • Involvement in disease

    Factor X deficiency

  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.

  • Post-translational
    modifications

    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
    The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Activated factor Xa heavy chain
    • F10
    • FA10_HUMAN
    • Stuart factor
    • Stuart-Prower factor
    see all

  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab108832 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent dilution.

    Images

    • ELISA: Factor X Human ELISA kit (ab108832)
      ELISA: Factor X Human ELISA kit (ab108832)

      Standard curve with background signal subtracted (duplicates; +/- SD).

    • ELISA: Factor X Human ELISA kit (ab108832)
      ELISA: Factor X Human ELISA kit (ab108832)

      Factor X measured in undiluted culture supernatants with MCF7 levels below level of detection (duplicates +/- SD).

    • ELISA: Factor X Human ELISA kit (ab108832)
      ELISA: Factor X Human ELISA kit (ab108832)

      Factor X measured in biological fluids (duplicates +/- SD). Serum and plasma were tested at 1/300-1/3000; other samples at 1/1-1/30.

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108832

    Protocols

    References

    ab108832 has not yet been referenced specifically in any publications.

    Publishing research using ab108832? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Question

    Dear Sirs, I am interested in buying a Factor X Human ELISA kit (ab108832), but I need to know Which is the epitope recognized by the antibody? In other words, Does the kit recognizes the inactive and active FX (FX and FXa)? Many thanks for your help.

    Best regards,

    Read More
    Answer

    Thank you for contacting us and your interest in our products.

    The Factor X Human ELISA kit, ab108832 enables both FX and FXa to detected in a range of biological samples.

    I hope this information has been helpful. If you have any further questions, please do not hesitate to let me know.

    Read More

    Question

    Hello,



    I have a question about ab108832 and I would appreciate a quick response, since I will perform the assay tomorrow.



    In your “Instructions for Use” it states in the assay summary that the biotin antibody needs to be incubated for 1 hour. However, in the assay method, it says that the biotin antibody needs to be incubated for 30 minutes.



    Could you please let me know whether this incubation needs to be 30 minutes or 1 hour?!



    Thanks in advance.



    Regards,

    Read More
    Answer

    Thank you for alerting us to this mistake in the protocol.

    I can confirm that the biotinylated antibody (page 10, step 5) should be incubated for 1 hour.

    The protocol will be up-dated as soon as possible.

    We wish you good luck with your experiments and a good day.

    Read More

    Question

    Dear sir, madam,



    Could you please let me know how long the fX Elisa Kits can be used? In other words, if I order an Elisa Kit now, what is the expiry date then?

    Ordering time is 1-2 weeks, as I understand?



    Regards,

    Read More
    Answer

    Thank you for your inquiry.

    ab108832 may be storedfor up tosix monthat 2 to 8°C or -20°C from the date of shipment.

    Thiskits as all our products is covered by our Abpromiseguarantee for six month afterdate of purchase:

    https://www.abcam.com/abpromise

    Opened diluents may be stored for up to 1 month at 2 to 8°C.

    Please store reconstituted reagents at -20°C or below. Opened unused strip wells may bereturned to the foil pouch with the desiccant pack, reseal along zip-seal and may be stored for up to 1 month in a vacuum desiccator.

    I can confirm that the lead time is currently 1-2 weeks.

    I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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