Human Factor X ELISA Kit (ab108832)
Key features and details
- Sensitivity: 1.5 ng/ml
- Range: 0.781 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Factor X ELISA Kit
See all Factor X kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 20 = 3.2% 2 20 = 3.4% 3 20 = 3.7% Inter-assay Sample n Mean SD CV% 1 20 = 6.2% 2 20 = 6.8% 3 20 = 7.2% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
= 1.5 ng/ml -
Range
0.781 ng/ml - 50 ng/ml -
Recovery
97.5 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human Factor Xin vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor X concentrations in plasma, serum, milk, urine, saliva, cerebrospinal fluid and cell culture supernatants.
A Factor X specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor X specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor X captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Biotinylated Antibody 1 x 60µl 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml Chromogen Substrate 1 x 7ml Factor X Microplate (12 x 8 well strips) 1 unit Factor X Standard 1 x 65ng Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. -
Tissue specificity
Plasma; synthesized in the liver. -
Involvement in disease
Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Activated factor Xa heavy chain
- Coagulation factor X
- F10
see all -
Database links
- Entrez Gene: 2159 Human
- Omim: 227600 Human
- SwissProt: P00742 Human
- Unigene: 361463 Human
Images
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ELISA: Factor X Human ELISA kit (ab108832)
Standard curve with background signal subtracted (duplicates; +/- SD).
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ELISA: Factor X Human ELISA kit (ab108832)Factor X measured in undiluted culture supernatants with MCF7 levels below level of detection (duplicates +/- SD).
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ELISA: Factor X Human ELISA kit (ab108832)Factor X measured in biological fluids (duplicates +/- SD). Serum and plasma were tested at 1/300-1/3000; other samples at 1/1-1/30.
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Typical Standard CurveRepresentative Standard Curve using ab108832
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab108832 has been referenced in 1 publication.
- Cohen CT et al. Production and control of coagulation proteins for factor X activation in human endothelial cells and fibroblasts. Sci Rep 10:2005 (2020). PubMed: 32029851