Human Factor XII ELISA Kit (ab108835)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Human Factor XII ELISA Kit
See all Factor XII kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5% Inter-assay Sample n Mean SD CV% Overall 7.1% -
Sample type
Cell culture supernatant, Milk, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.02 ng/ml -
Range
6.25 ng/ml - 100 ng/ml -
Recovery
98 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Factor XII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor XII concentrations in plasma, serum, milk, urine and cell culture supernatants.
A Factor XII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XII captured in plate.
Get results in 90 minutes with Human Factor XII ELISA Kit (ab192144) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests Factor XII Standard 2 vials 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 40X Biotinylated Human Factor XII Antibody 1 x 150µl Chromogen Substrate 1 x 8ml Factor XII Microplate (12 x 8 well strips) 1 unit Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. -
Involvement in disease
Factor XII deficiency
Hereditary angioedema 3 -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 fibronectin type-I domain.
Contains 1 fibronectin type-II domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsFactor XII is activated by kallikrein in alpha-factor XIIa, which is then further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of the NH2-terminal heavy chain (Coagulation factor XIIa heavy chain) and the COOH-terminal light chain (Coagulation factor XIIa light chain), connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, a light chain (Beta-factor XIIa part 2), corresponding to the COOH-terminal light chain (Coagulation factor XIIa light chain) and a nonapeptide (Beta-factor XIIa part 1).
O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Beta-factor XIIa part 1
- Beta-factor XIIa part 2
- Coagulation factor XII
see all -
Database links
- Entrez Gene: 2161 Human
- Omim: 610619 Human
- SwissProt: P00748 Human
- Unigene: 1321 Human
Associated products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab108835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent dilution. |
Images
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ELISA: Factor XII Human ELISA kit (ab108835)Standard curve with background signal subtracted (duplicates; +/- SD).
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ELISA: Factor XII Human ELISA kit (ab108835)Factor XII measured in undiluted culture supernatants, MCF-7 levels below level of detection (duplicates +/- SD).
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ELISA: Factor XII Human ELISA kit (ab108835)Factor XII measured in biological fluids (duplicates +/- SD). Human plasma and serum were tested in the dilution range of 1/100-1/4000; milk, saliva, CSF and urine were diluted 1/1-1/300.
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Typical Standard CurveRepresentative Standard Curve using ab108835.
Protocols
References
This product has been referenced in:
- Liang Y et al. Coagulation cascade and complement system in systemic lupus erythematosus. Oncotarget 9:14862-14881 (2018). Read more (PubMed: 29599912) »
