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  1. Link

    human-factor-xii-elisa-kit-ab108835.pdf

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Cardiovascular Blood Fibrinolysis / Thrombolysis
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Human Factor XII ELISA Kit (ab108835)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (2)References (1)

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ELISA: Factor XII Human ELISA kit (ab108835)
  • ELISA: Factor XII Human ELISA kit (ab108835)
  • ELISA: Factor XII Human ELISA kit (ab108835)
  • Typical Standard Curve

Key features and details

  • Sensitivity: 0.02 ng/ml
  • Range: 6.25 ng/ml - 100 ng/ml
  • Sample type: Cell culture supernatant, Milk, Plasma, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Get results in 90 minutes with a SimpleStep ELISA® kit

Overview

  • Product name

    Human Factor XII ELISA Kit
    See all Factor XII kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Sample type

    Cell culture supernatant, Milk, Urine, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.02 ng/ml
  • Range

    6.25 ng/ml - 100 ng/ml
  • Recovery

    98 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Factor XII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor XII concentrations in plasma, serum, milk, urine and cell culture supernatants.


    A Factor XII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XII captured in plate.


    Get results in 90 minutes with Human Factor XII ELISA Kit (ab192144) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    40X Biotinylated Human Factor XII Antibody 1 x 150µl
    Chromogen Substrate 1 x 7ml
    Factor XII Microplate (12 x 8 well strips) 1 unit
    Factor XII Standard 2 vials
    Sealing Tapes 3 units
    Stop Solution 1 x 11ml
  • Research areas

    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cardiovascular
    • Blood
    • Coagulation
    • Regulatory
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Blood coagulation ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cardiovascular ELISA kits
  • Function

    Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
  • Involvement in disease

    Factor XII deficiency
    Hereditary angioedema 3
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 fibronectin type-I domain.
    Contains 1 fibronectin type-II domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    Factor XII is activated by kallikrein in alpha-factor XIIa, which is then further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of the NH2-terminal heavy chain (Coagulation factor XIIa heavy chain) and the COOH-terminal light chain (Coagulation factor XIIa light chain), connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, a light chain (Beta-factor XIIa part 2), corresponding to the COOH-terminal light chain (Coagulation factor XIIa light chain) and a nonapeptide (Beta-factor XIIa part 1).
    O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P00748 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Beta-factor XIIa part 1
    • Beta-factor XIIa part 2
    • Coagulation factor XII
    • Coagulation factor XIIa heavy chain
    • Coagulation factor XIIa light chain
    • F12
    • FA12_HUMAN
    • HAF
    • Hageman factor
    see all
  • Database links

    • Entrez Gene: 2161 Human
    • Omim: 610619 Human
    • SwissProt: P00748 Human
    • Unigene: 1321 Human

    Associated products

    • SimpleStep ELISA kits

      • Human Factor XII ELISA Kit (ab192144)

    Images

    • ELISA: Factor XII Human ELISA kit (ab108835)
      ELISA: Factor XII Human ELISA kit (ab108835)

      Standard curve with background signal subtracted (duplicates; +/- SD).

    • ELISA: Factor XII Human ELISA kit (ab108835)
      ELISA: Factor XII Human ELISA kit (ab108835)

      Factor XII measured in undiluted culture supernatants, MCF-7 levels below level of detection (duplicates +/- SD).

    • ELISA: Factor XII Human ELISA kit (ab108835)
      ELISA: Factor XII Human ELISA kit (ab108835)

      Factor XII measured in biological fluids (duplicates +/- SD). Human plasma and serum were tested in the dilution range of 1/100-1/4000; milk, saliva, CSF and urine were diluted 1/1-1/300.

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108835.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab108835? Please let us know so that we can cite the reference in this datasheet.

    ab108835 has been referenced in 1 publication.

    • Liang Y  et al. Coagulation cascade and complement system in systemic lupus erythematosus. Oncotarget 9:14862-14881 (2018). PubMed: 29599912

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Can these reactions be done at room temperature instead of 37C? Can I just analyze the end points or will this be less accurate?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 13 2012

    Answer

    Thank you for your call last week and for your patience. I apologize for the extended delay in getting this information for you.

    I've just received a reply from the lab, and these kits can be analyzed at room temperature. The OD might be slightly lower than at 37C but the kinetics should not be affected. If you warm your samples to 37C prior to analysis and then read the plate at room temperature, there should not be a noticeable effect. We would still recommend analyzing the kinetic data as opposed to an end point analysis.

    I hope this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you. I'll be happy to help.

    Read More

    Abcam Scientific Support

    Answered on Apr 13 2012

    Question

    Interested in ordering these kits.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 30 2012

    Answer

    Thank you for your call today.

    As we discussed, you can email mailto:us.sales@abcam.com for information regarding bulk purchases.

    Please let me know if you have any questions or if there is anything else that we can do for you.

    Read More

    Abcam Scientific Support

    Answered on Mar 30 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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