Overview

  • Product name
    Human Factor XII ELISA Kit
    See all Factor XII kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.02 ng/ml
  • Range
    6.25 ng/ml - 100 ng/ml
  • Recovery

    98 %

  • Assay time
    4h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Factor XII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor XII concentrations in plasma, serum, milk, urine and cell culture supernatants.


    A Factor XII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XII captured in plate.


    Get results in 90 minutes with Human Factor XII ELISA Kit (ab192144) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    40X Biotinylated Human Factor XII Antibody 1 x 150µl
    Chromogen Substrate 1 x 8ml
    Factor XII Microplate (12 x 8 well strips) 1 unit
    Factor XII Standard 2 vials
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
  • Involvement in disease
    Factor XII deficiency
    Hereditary angioedema 3
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 fibronectin type-I domain.
    Contains 1 fibronectin type-II domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    Factor XII is activated by kallikrein in alpha-factor XIIa, which is then further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of the NH2-terminal heavy chain (Coagulation factor XIIa heavy chain) and the COOH-terminal light chain (Coagulation factor XIIa light chain), connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, a light chain (Beta-factor XIIa part 2), corresponding to the COOH-terminal light chain (Coagulation factor XIIa light chain) and a nonapeptide (Beta-factor XIIa part 1).
    O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Beta-factor XIIa part 1
    • Beta-factor XIIa part 2
    • Coagulation factor XII
    • Coagulation factor XIIa heavy chain
    • Coagulation factor XIIa light chain
    • F12
    • FA12_HUMAN
    • HAF
    • Hageman factor
    see all
  • Database links

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab108835 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent dilution.

    Images

    • ELISA: Factor XII Human ELISA kit (ab108835)
      ELISA: Factor XII Human ELISA kit (ab108835)

      Standard curve with background signal subtracted (duplicates; +/- SD).

    • ELISA: Factor XII Human ELISA kit (ab108835)
      ELISA: Factor XII Human ELISA kit (ab108835)

      Factor XII measured in undiluted culture supernatants, MCF-7 levels below level of detection (duplicates +/- SD).

    • ELISA: Factor XII Human ELISA kit (ab108835)
      ELISA: Factor XII Human ELISA kit (ab108835)

      Factor XII measured in biological fluids (duplicates +/- SD). Human plasma and serum were tested in the dilution range of 1/100-1/4000; milk, saliva, CSF and urine were diluted 1/1-1/300.

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108835.

    Protocols

    References

    ab108835 has not yet been referenced specifically in any publications.

    Publishing research using ab108835? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    2 reviews or Q&A

    10
    Votes: Highest First

    Can these reactions be done at room temperature instead of 37C? Can I just analyze the end points or will this be less accurate?

    Thank you for your call last week and for your patience. I apologize for the extended delay in getting this information for you.

    I've just received a reply from the lab, and these kits can be analyzed at room temperature. The OD might be slig...

    Read More

    Interested in ordering these kits.

    Thank you for your call today.

    As we discussed, you can email mailto:us.sales@abcam.com for information regarding bulk purchases.

    Please let me know if you have any questions or if there is anything else that we can do for you.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up