Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.1 ng/ml
- Range: 0.23 ng/ml - 15 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Factor XII ELISA Kit
See all Factor XII kits
Intra-assay Sample n Mean SD CV% plasma 8 3% Inter-assay Sample n Mean SD CV% plasma 3 5.5%
Sample typeCell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range0.23 ng/ml - 15 ng/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 93 88% - 98% Urine 107 104% - 110% Serum 109 98% - 118% Hep Plasma 105 100% - 109% EDTA Plasma 120 117% - 123% Cit plasma 110 108% - 116%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
As of April 2020, Human Factor XII ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.
Human Factor XII ELISA Kit (ab192144) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor XII protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Factor XII with 0.1 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Factor XII is a 80 kDa plasma serine protease involved in the coagulation cascade. Factor XII is activated by proteolysis and the product, Factor XIIa, goes on to activate Factor XI and prekallikrein in in vitro models of blood cogulation. Its involvement in the beginning of the coagulation cascade is only seen in in vitro studies, and is thought to have no effect on coagulation initiation in vivo. While Factor XII deficiency in humans is asymptomatic, Factor XII plays a role in later stages of clot formation. Deficiency of Factor XII affects the in vitro measurements of partial thromboplastin time in human blood, and its absence decreases the susceptibility of mice to thrombosis.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human Factor XII Capture Antibody 1 x 600µl 10X Human Factor XII Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Human Factor XII Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionFactor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
Involvement in diseaseFactor XII deficiency
Hereditary angioedema 3
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 fibronectin type-I domain.
Contains 1 fibronectin type-II domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain.
modificationsFactor XII is activated by kallikrein in alpha-factor XIIa, which is then further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of the NH2-terminal heavy chain (Coagulation factor XIIa heavy chain) and the COOH-terminal light chain (Coagulation factor XIIa light chain), connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, a light chain (Beta-factor XIIa part 2), corresponding to the COOH-terminal light chain (Coagulation factor XIIa light chain) and a nonapeptide (Beta-factor XIIa part 1).
O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.
- Information by UniProt
- Beta-factor XIIa part 1
- Beta-factor XIIa part 2
- Coagulation factor XII
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
The Factor XII standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:6,600, plasma (EDTA) 1:1,600, plasma (citrate) 1:1,600 and plasma (heparin) 1:8,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 25.0 µg/mL in serum, 21.7 µg/mL in plasma (EDTA), 21.0 µg/mL in plasma (citrate), and 32.1 µg/mL in plasma (heparin).
The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 60.7 ng/mL in urine 12.5%.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 30.4 µg/mL with a range of 21.8 – 40.3 µg/mL.
ab192144 has not yet been referenced specifically in any publications.