Human Factor XIII ELISA Kit (ab108836)

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Overview

  • Product name

    Human Factor XIII ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8.7%

  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    = 2.1 ng/ml

  • Range

    5 ng/ml - 80 ng/ml

  • Recovery

    99 %

  • Assay time

    4h 0m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s Factor XIII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of factor XIII in plasma, serum, milk, urine, saliva, and cell culture supernatants.


    A Factor XIII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XIII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XIII captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    60X Biotinylated Human Factor XIII Antibody 1 x 100µl
    Chromogen Substrate 1 x 8ml
    Factor XIII Microplate (12 x 8 well strips) 1 unit
    Factor XIII Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml

  • Research areas

  • Function

    Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.

  • Involvement in disease

    Factor XIII subunit A deficiency

  • Sequence similarities

    Belongs to the transglutaminase superfamily. Transglutaminase family.

  • Post-translational
    modifications

    The activation peptide is released by thrombin.

  • Cellular localization

    Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
  • Information by UniProt

  • Alternative names

    • Coagulation factor XIII A chain
    • Coagulation factor XIIIa
    • F13A_HUMAN
    • F13a1
    • Protein-glutamine gamma-glutamyltransferase A chain
    • Transglutaminase A chain
    see all

  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab108836 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent dilution.

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108836

    Protocols

    References

    This product has been referenced in:

    • Peltier J  et al. Activation peptide of the coagulation factor XIII (AP-F13A1) as a new biomarker for the screening of colorectal cancer. Clin Proteomics 15:15 (2018). Read more (PubMed: 29657559) »
    • Liang Y  et al. Coagulation cascade and complement system in systemic lupus erythematosus. Oncotarget 9:14862-14881 (2018). Read more (PubMed: 29599912) »
    See all 2 Publications for this product

    Publishing research using ab108836? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Question

    we would like you to specify what the ELISA antibody binds to, please read the below email.

    Read More
    Answer

    Thank you for contacting us.

    The kit using different antibodies, but the binding site is not mapped. Since they both polyclonal antibody, it may have multiple binding sites for protein.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Does this kit detect both the Factor XIIIA and B?

    Read More
    Answer

    Thank you for contacting us.


    Yes, the kit will detect both the A and B forms.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    How can I get a discount from ordering in bulk?

    Read More
    Answer

    Thanks for your enquiry!

    Please contact our bulk order specialist, Andrea Gray at mailto:Andrea.Gray@abcam.com, regarding discounts on bulk order purchases.

    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Question

    The OD's for the standard curve were very low- the highest standard measured at 0.21. Background was 0.046. Samples read around 0.1 (double the background). This seems very low, do you agree? After the streptavidin-peroxidase incubation, the plates were washed and left at room temperature for 45 min. Could this have caused the low signal? Do you have any suggestions to increase the signal?
    Also, I'm expecting that my plasma samples will have very low levels of the analyte. Can I dilute them less than 1:1000 (which the protocol instructs to do)? I would like to dilute some of my samples only 1:100, and others 1:300-1:500. Will this interfere with the assay? Will the higher concentration of chelating agent (EDTA) interfere?
    Thanks!

    Read More
    Answer

    Thank you for your call last week and for your patience while I've been in touch with the lab regarding your questions.

    I've heard back from my contact at the lab, and she agrees that the OD values are lower than expected. An OD of 1.5-2.0 would be expected for the highest standard.She also believes this could have been caused by the breakduring the protocol, and she recommends repeating the experimentonce more without the 45 minute break, and compare the results.


    It is also fine to optimize the dilution of the samples, and 1:1000 is only a suggested startingdilution. it would be a good idea to test various dilutions to find the optimal dilution factor for your plasma samples.

    I hope this information will be useful. Please keep me updated with any progress, and let me know about the results after repeating the assay. If there is anything else that we can do for you, I will be happy to help you furhter.

    Read More

    Question

    needs an antibody/ELISA kit which would recongize the first 37 amino acids of the Factor XIII

    Read More
    Answer

    Thank you for your call the other day.

    I am happy to confirm that the kit ab108836 against the human Factor XIII will recognize the full length protein as well as the fragment of the 37 first amino acids. I have just received the confirmation from the laboratory.

    I have checked also for the antibodies against Factor XIII:There is none which is specifically targeted to this region. We have several polyclonal antibodies which are against the full length protein. They might recognize the fragment as well, we can however not be sure. Please let me know if you would like me to investigate those further.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Which cleavage products will ab108836 detect? There are 3, 80kDa, 56kDa and 24kDa.

    Read More
    Answer

    This ELISA kit will recognize factor XIIIa and factor XIII.  I hope this is helpful. Please contact me again if you have any further questions.  

    Read More

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