Key features and details
- One-wash 90 minute protocol
- Sensitivity: 398 pg/ml
- Range: 1.56 ng/ml - 100 ng/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman FAK ELISA Kit
Intra-assay Sample n Mean SD CV% HeLa extract 5 5.9% Inter-assay Sample n Mean SD CV% HeLa extract 3 9.7%
Sample typeCell culture extracts, Tissue Extracts
Assay typeSandwich (quantitative)
Range1.56 ng/ml - 100 ng/ml
Sample specific recovery Sample type Average % Range Cell culture media 100 99% - 100% Extraction Buffer 101 100% - 103%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Human FAK ELISA Kit (ab187395) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FAK protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FAK with 398 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Focal Adhesion Kinase (FAK) is a non-receptor protein-tyrosine kinase that is implicated in signaling pathways involved in cell motility, proliferation and apoptosis. FAK is widely expressed in adult tissues as well as being critical during development (angiogenesis, heart and nervous system development). Post-translational modifications include phosphorylation at residue Tyrosine 397. Phosphorylation at this residue promotes interaction with SRC family members and signal transduction (including activation of AKT, MAPK and Rho GTPase cascades); dephosphorylation at this residue promotes focal adhesion disassembly. FAK overexpression and increased kinase activity is observed in many types of cancer.
The standard is affinity purified.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human FAK Capture Antibody 1 x 600µl 10X Human FAK Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 200X SDS 1 x 1ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human FAK Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
Cellular localizationCell Membrane, Cytoplasmic and Nuclear
- FADK 1
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
Interpolated values of FAK are plotted for the indicated cell lines based on an extract load of 125 µg/mL.
The concentrations of FAK protein were interpolated from the FAK standard curve and corrected for sample dilution relative to extract load of 62.5 µg/mL. The mean FAK concentration was determined to be 44 ng/mL in HeLa extract at 62.5 µg/mL.
ab187395 has been referenced in 1 publication.
- Jung S et al. Influence of Implant Material and Surface on Mode and Strength of Cell/Matrix Attachment of Human Adipose Derived Stromal Cell. Int J Mol Sci 21:N/A (2020). PubMed: 32526920