Overview

  • Product name

    Human FAP ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Serum, Plasma, Cell culture media
  • Assay type

    Sandwich
  • Sensitivity

    12 pg/ml
  • Range

    12 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 91.52 84% - 100%
    Plasma 86.5 82% - 98%
    Cell culture media 91.09 83% - 99%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam's FAP Human ELISA Kit (ab193701) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FAP in serum, plasma, cell culture supernatants. (UniprotID: Q12884)


    This assay employs an antibody specific for human FAP coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures FAP present in the samples. The wells are washed and biotinylated anti-human FAP antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of FAP bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated Human FAP detection antibody (lyophilized) 2 vials
    Human FAP Standards (lyophilized) 2 vials
    Pre-coated Human FAP Microplate (12 Strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Function

    In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
  • Tissue specificity

    Fibroblast specific.
  • Sequence similarities

    Belongs to the peptidase S9B family.
  • Post-translational
    modifications

    N-glycosylated.
    The N-terminus may be blocked.
  • Cellular localization

    Cell membrane. Cell projection, lamellipodium membrane. Cell projection, invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
  • Information by UniProt
  • Alternative names

    • 170 kDa melanoma membrane-bound gelatinase
    • Dipeptidyl peptidase FAP
    • DPPIV
    • FAP
    • FAPA
    • FAPalpha
    • Fibroblast activation protein alpha
    • Gelatine degradation protease FAP
    • Integral membrane serine protease
    • Post proline cleaving enzyme
    • SEPR_HUMAN
    • Seprase
    • Serine integral membrane protease
    • SIMP
    • Surface expressed protease
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

Protocols

References

ab193701 has not yet been referenced specifically in any publications.

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