For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
I received your e-mail
I prefer [CD95 Human ELISA Kit (ab100513) and Fas Ligand Human ELISA Kit (ab100515)] because of the high sensitivity, and as I mentioned, I deal with soluble Fas and FasL in human serum and saliva.
My question; If I use [CD95 Human ELISA Kit (ab100513) and Fas Ligand Human ELISA Kit (ab100515)] kits, the results represent soluble Fas and FasL or both membrane bound and soluble Fas and FasL in human serum and saliva?
Thank you for your patiency.
Asked on Sep 07 2012
Thank you for your reply.
I can confirm that these kits will detect both soluble and membrane bound Fas and fas ligand.
In order to prevent membrane bound protein to interfere with your results, I suggest to spin down the saliva samples to get rid of all cells while taking care not to generate cell debris.
Plasma is is in generally cell free and therefore should not contain any membrane bound proteins.
I am sorry that I could not be of more help and hope this information is nevertheless helpful.
Answered on Sep 07 2012