Human FAS ELISA Kit (CD95) (ab119571)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 3 pg/ml
    • Range: 31.2 pg/ml - 2000 pg/ml
    • Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    Overview

    • Product name

      Human FAS ELISA Kit (CD95)
      See all Fas kits

    • Detection method

      Colorimetric

    • Sample type

      Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      < 3 pg/ml

    • Range

      31.2 pg/ml - 2000 pg/ml

    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Serum 31.2pg/ml - 2000pg/ml
      Other biological fluids 31.2pg/ml - 2000pg/ml

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Human

    • Product overview

      Abcam’s Human FAS (CD95) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human FAS (CD95) in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).


      A FAS (CD95) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for FAS (CD95) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human FAS (CD95) amount of sample captured in plate.


      Get results in 90 minutes with Human FASLG Receptor ELISA Kit (CD95) (ab183360) from our SimpleStep ELISA® range.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-Human FAS Antibody Microplate (12 x 8 wells) 1 unit
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
      Biotinylated anti-Human FAS antibody 1 x 130µl
      Lyophilized recombinant Human FAS standard 2 x 10ng
      Plate Seal 1 x 4 units
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Amber Bottle 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml

    • Research areas

    • Function

      Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).

    • Tissue specificity

      Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.

    • Involvement in disease

      Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.

    • Sequence similarities

      Contains 1 death domain.
      Contains 3 TNFR-Cys repeats.

    • Domain

      Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.

    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt

    • Alternative names

      • ALPS 1A
      • ALPS1A
      • APO 1
      • Apo 1 antigen
      • APO 1 cell surface antigen
      • Apo-1 antigen
      • APO1
      • Apo1 antigen
      • APO1 cell surface antigen
      • Apoptosis antigen 1
      • Apoptosis mediating surface antigen FAS
      • Apoptosis-mediating surface antigen FAS
      • APT 1
      • APT1
      • CD 95
      • CD 95 antigen
      • CD95
      • CD95 antigen
      • Delta Fas
      • Delta Fas/APO 1/CD95
      • Delta Fas/APO1/CD95
      • Fas
      • Fas (TNF receptor superfamily, member 6)
      • FAS 1
      • FAS 827dupA
      • Fas AMA
      • FAS Antigen
      • Fas cell surface death receptor
      • FAS1
      • FASLG receptor
      • FASTM
      • sFAS
      • Surface antigen APO1
      • TNF receptor superfamily, member 6
      • TNFRSF 6
      • TNFRSF6
      • TNR6_HUMAN
      • Tumor necrosis factor receptor superfamily member 6
      see all

    • Database links

      Associated products

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab119571.

      References (1)

      Publishing research using ab119571? Please let us know so that we can cite the reference in this datasheet.

      ab119571 has been referenced in 1 publication.

      • Jiang W  et al. Apoptosis in cerebrospinal fluid as outcome predictors in severe traumatic brain injury: An observational study. Medicine (Baltimore) 99:e20922 (2020). PubMed: 32590803

      Customer reviews and Q&As

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      Question

      My search deals with human Fas, Fas ligand and Bcl-2 in saliva and serum as a sample.
      In your company, I found these kits;
      1- FAS Human ELISA Kit (ab119571)
      2- CD95 Human ELISA Kit (ab100513)
      3- Fas Ligand Human ELISA Kit - 1 x 96 Well Plate (ab100515)
      4- Bcl-2 Human ELISA Kit (with color giving dyes) (ab119506)
      Please, your opinion about these kits.
      What is the best (ab119571) and (ab100513) in regard to my search?

      Read More
      Answer

      Thank you for contacting us.

      The best kit will depend on the amount of FAS (CD95) that you are expecting to detect in your samples. The kit ab100513 produces a standard curve that is linear for lower concentrations of FAS (CD95) than ab119571. Both of these kits will be guaranteed for use on human serum samples, but have not been tested on saliva samples. In theory, they should work for saliva, but the sample dilution will need to be optimized.

      I hope this helps, please let me know if you need any additional information or assistance.

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