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If I use [CD95 Human ELISA Kit (ab100513) and Fas Ligand Human ELISA Kit (ab100515)] kits, the results represent soluble Fas and FasL or both membrane bound and soluble Fas and FasL in human serum and saliva?
Asked on Sep 07 2012
I can confirm that these kits will detect both soluble and membrane bound Fas and fas ligand.
In order to prevent membrane bound protein to interfere with your results, I suggest to spin down the saliva samples to get rid of all cells while taking care not to generate cell debris.
Plasma is is in generally cell free and therefore should not contain any membrane bound proteins.
Answered on Sep 07 2012