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Please, I want to ask if you can send to me the optical density (OD) for each standard after serial dilution and standard curve of these readings and its equation;
stock solution (S1) of 1000 pg/ml= OD ?
S2 (333.3 pg/ml)= OD?
S3 (111.1 pg/ml)=OD?
S4 (37.04 pg/ml)=OD?
S5 (12.35 pg/ml)=OD?
S6 (4.12 pg/ml)=OD?
S7 (1.37 pg/ml)=OD?
S8 (0 pg/ml)=OD?
My samples are serum and saliva; I want to know your opinion and advice about sample dilution.
If you recommend the dilution, what should I use for saliva samples Assay Diluent A (Item D) or Assay Diluent B (Item E).
I want to know the expired date of this kit.
Asked on Mar 06 2013
Unfortunately, the OD standard is never twice the same, therefore, it is so important to run a fresh standard curve for EVERY assay. The OD needs to be measured, it cannot be not calculated.
How to analyze the data is described under section 8, page 11 of our protocol booklet (see attachment).
As we haven't tested saliva samples, we cannot make a proper suggestion. I would suggest to test this yourself.
According to the protocol booklet, the storage and handling is described in section 4:
ab100515 may be stored for up to 6 months at 2 to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution.
Opened Microplate Wells or reagents may be store for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one
year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles
Answered on Mar 06 2013