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Cell Biology Apoptosis Extracellular Signals Death Ligands
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Human FAS Ligand ELISA Kit (CD95L) (ab45892)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (2)References (1)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 12 pg/ml
    • Range: 62.5 pg/ml - 2000 pg/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Protein
    Recombinant human Fas Ligand protein (Active) (ab109359)

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    Overview

    • Product name

      Human FAS Ligand ELISA Kit (CD95L)
      See all Fas Ligand kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Sample A 40 588.4pg/ml 22.6 3.8%
      Sample B 35 302.2pg/ml 14.6 4.5%
    • Sample type

      Cell culture supernatant, Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 12 pg/ml
    • Range

      62.5 pg/ml - 2000 pg/ml
    • Assay time

      3h 45m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s Human FAS Ligand in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of FAS Ligand in Human serum, plasma, buffered solutions and cell culture media.

      A monoclonal antibody specific for FAS Ligand has been coated onto the wells of the microtiter strips provided. Samples, including standards of known FAS Ligand concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for FAS Ligand are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of FAS Ligand present in the samples. 

      This kit will recognize both endogenous and recombinant Human FAS Ligand.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests 2 x 96 tests
      10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml
      200X Wash Buffer White 1 x 10ml 2 x 10ml
      Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
      Biotinylated anti-FAS Ligand Red 1 x 400µl 2 x 400µl
      Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
      Control Silver 2 vials 4 vials
      FAS Ligand Microplate (12 x 8 well strips) 1 unit 2 units
      FAS Ligand Standard (Lyophilized) Yellow 2 vials 4 vials
      HRP Diluent Red 1 x 12ml 1 x 23ml
      Stop Reagent Black 1 x 11ml 2 x 11ml
      Streptavidin-HRP 2 x 5µl 4 x 5µl
    • Research areas

      • Cell Biology
      • Apoptosis
      • Extracellular Signals
      • Death Ligands
      • Cell Biology
      • Apoptosis
      • Receptors
      • Death Receptors
      • Immunology
      • Innate Immunity
      • Cytokines
      • TNF Superfamily
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Cell survival & death
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Apoptosis marker and proteins ELISA kits
    • Relevance

      Fas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumours. Tumour cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system.
    • Cellular localization

      Cell Membrane (single-pass type II membrane protein) and Secreted
    • Alternative names

      • Apoptosis (APO 1) antigen ligand 1
      • Apoptosis antigen ligand
      • APT1LG1
      • APTL
      • CD178
      • CD95 L
      • CD95 ligand
      • CD95L
      • CD95L protein
      • Fas antigen ligand
      • FASL
      • FASLG
      • TNF superfamily member 6
      • TNFSF6
      • Tumor necrosis factor (ligand) superfamily member 6
      • Tumor necrosis factor ligand superfamily member 6
      see all
    • Database links

      • Entrez Gene: 356 Human
      • Omim: 134638 Human
      • SwissProt: P48023 Human

      Associated products

        Images

        • Typical Standard Curve
          Typical Standard Curve

          Representative standard curve using ab45892.

        Protocols

        • Human Fas Ligand ELISA kit
        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • SDS download

        • Datasheet download

          Download

        References (1)

        Publishing research using ab45892? Please let us know so that we can cite the reference in this datasheet.

        ab45892 has been referenced in 1 publication.

        • Qiu M  et al. Transforming growth factor ß1 and Fas ligand synergistically enhance immune tolerance in dendritic cells in liver transplantation. J Surg Res 218:180-193 (2017). PubMed: 28985848

        Customer reviews and Q&As

        Show All Reviews Q&A
        Submit a review Submit a question

        1-2 of 2 Abreviews or Q&A

        Question

        Please,

        My samples are serum and saliva; and i deal with soluble human Fas and Fas Ligand.

        My question, what are the differences between [Fas Ligand Human ELISA Kit (ab45892) and Fas Ligand Human ELISA Kit (ab100515)] and what are the differences between [CD95 Human ELISA Kit (ab45922) and CD95 Human ELISA Kit (ab100513)].

        As I mentioned, I deal with soluble human Fas and Fas Ligand, not with membrane bound Fas and Fas Ligand.

        Please, I am waiting

        Thank you

        Read More

        Abcam community

        Verified customer

        Asked on Sep 05 2012

        Answer

        Thank you for your enquiry.

        I have reviewed the details on the datasheets for these kits. For the Fas ligand kits:

        ab45892 Fas Ligand Human ELISA Kit
        ab100515 Fas Ligand Human ELISA Kit

        Sensitivities are different:ab100515 < 2 pg/ml, ab45892 < 12 pg/ml

        Detection ranges are different:ab10515 1.37 pg/ml - 1000 pg/ml,ab45892 62.5 pg/ml - 2000 pg/ml

        For the CD95 kits:
        ab45922 CD95 Human ELISA Kit
        ab100513 CD95 Human ELISA Kit

        Again, sensitivities are different: ab100513 < 5 pg/ml, ab45922 < 47 pg/ml
        Detection ranges are different:ab45922 93.75 pg/ml - 3000 pg/ml, ab100513 2.74 pg/ml - 2000 pg/ml

        These are the main differences you may need to take into consideration. However, I can recommend to review the datasheets fullyfor further information and otherdifferences.

        I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

        Read More

        Abcam Scientific Support

        Answered on Sep 05 2012

        Question

        Please,

        My samples are serum and saliva; and i deal with soluble human Fas and Fas Ligand.

        My question, what are the differences between [Fas Ligand Human ELISA Kit (ab45892) and Fas Ligand Human ELISA Kit (ab100515)] and what are the differences between [CD95 Human ELISA Kit (ab45922) and CD95 Human ELISA Kit (ab100513)].

        As I mentioned, I deal with soluble human Fas and Fas Ligand, not with membrane bound Fas and Fas Ligand.

        Please, I am waiting…

        Thank you

        Read More

        Abcam community

        Verified customer

        Asked on Sep 05 2012

        Answer

        Thank you for your inquiry.

        These ELISA kits are from two different sources but they are similar.

        I recommend todetermine which is the best foryour needs from the datasheet and protocol information. Please take not of the different ranges these kits are suitable for. I suggest to choose the kits that are most suitable for the amounts of Fas ligand and CD95 you are expecting in your samples.

        I believe that all these kits are suitable for un-bound (free) Fas ligand and CD95 since for ELISA the cells are lysed and ELISA is not a cell based assay.

        I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

        Read More

        Abcam Scientific Support

        Answered on Sep 05 2012

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        For licensing inquiries, please contact partnerships@abcam.com

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