Human FAS Ligand ELISA Kit (CD95L) (ab45892)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Human FAS Ligand ELISA Kit (CD95L)
See all Fas Ligand kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Sample A 40 588.4pg/ml 22.6 3.8% Sample B 35 302.2pg/ml 14.6 4.5% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 12 pg/ml -
Range
62.5 pg/ml - 2000 pg/ml -
Assay time
3h 45m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human FAS Ligand in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of FAS Ligand in Human serum, plasma, buffered solutions and cell culture media.
A monoclonal antibody specific for FAS Ligand has been coated onto the wells of the microtiter strips provided. Samples, including standards of known FAS Ligand concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for FAS Ligand are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of FAS Ligand present in the samples.
This kit will recognize both endogenous and recombinant Human FAS Ligand.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-FAS Ligand Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml Control Silver 2 vials 4 vials FAS Ligand Microplate (12 x 8 well strips) 1 unit 2 units FAS Ligand Standard (Lyophilized) Yellow 2 vials 4 vials HRP Diluent Red 1 x 23ml 1 x 23ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
Research areas
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Relevance
Fas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumours. Tumour cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system. -
Cellular localization
Cell Membrane (single-pass type II membrane protein) and Secreted -
Alternative names
- Apoptosis (APO 1) antigen ligand 1
- Apoptosis antigen ligand
- APT1LG1
see all -
Database links
- Entrez Gene: 356 Human
- Omim: 134638 Human
- SwissProt: P48023 Human
Applications
Our Abpromise guarantee covers the use of ab45892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Typical Standard CurveRepresentative standard curve using ab45892.
Protocols
References
This product has been referenced in:
- Qiu M et al. Transforming growth factor ß1 and Fas ligand synergistically enhance immune tolerance in dendritic cells in liver transplantation. J Surg Res 218:180-193 (2017). Read more (PubMed: 28985848) »
