Human Ferritin ELISA Kit (ab108837)
Key features and details
- Sensitivity: 0.5 ng/ml
- Range: 0.781 ng/ml - 50 ng/ml
- Sample type: Cell Lysate, Cerebral Spinal Fluid, Milk, Plasma, Serum, Tissue, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Ferritin ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.8% Inter-assay Sample n Mean SD CV% Overall 10% -
Sample type
Milk, Urine, Serum, Plasma, Tissue, Cell Lysate, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.5 ng/ml -
Range
0.781 ng/ml - 50 ng/ml -
Recovery
95 %
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Assay time
4h 0m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Predicted to work with: Monkey -
Product overview
Abcam’s Ferritin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ferritin concentrations in plasma, serum, milk, cerebrospinal fluid and cell culture supernatants.
A Ferritin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ferritin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ferritin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 20ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Ferritin Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Ferritin Microplate (12 x 8 well strips) 1 unit Ferritin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. -
Sequence similarities
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. - Information by UniProt
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Alternative names
- Cell proliferation-inducing gene 15 protein
- Ferritin H subunit
- Ferritin heavy chain
see all -
Database links
- Entrez Gene: 2512 Human
- Entrez Gene: 2495 Human
- Omim: 608847 Human
- SwissProt: P02792 Human
- SwissProt: P02794 Human
- Unigene: 524910 Human
- Unigene: 645560 Human
Images
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Human Ferritin measured in biological fluids showing quantity (pg) per mL of tested sample. Serum and plasma samples were diluted 10-20 fold. Milk, urine and PBMC supernatant were diluted 1-4 fold.
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Human Ferritin standard curve: mean of duplicates (+/- SD)
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Representative Standard Curve using ab108837
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab108837 has been referenced in 16 publications.
- Wu J et al. The interaction between STING and NCOA4 exacerbates lethal sepsis by orchestrating ferroptosis and inflammatory responses in macrophages. Cell Death Dis 13:653 (2022). PubMed: 35902564
- Martínez-Soto JM et al. Increased serum ferritin is associated with oxidized low-density lipoprotein in prediabetes patients: A pilot study. Heliyon 7:e06720 (2021). PubMed: 33912708
- Hu Y et al. Clinical Characteristics, Iron Metabolism and Neuroinflammation: New Insight into Excessive Daytime Sleepiness in Parkinson's Disease. Neuropsychiatr Dis Treat 17:2041-2051 (2021). PubMed: 34188474
- Lian TH et al. Tremor-Dominant in Parkinson Disease: The Relevance to Iron Metabolism and Inflammation. Front Neurosci 13:255 (2019). PubMed: 30971879
- Choi EK et al. Ferroportin disease mutations influence manganese accumulation and cytotoxicity. FASEB J 33:2228-2240 (2019). PubMed: 30247984