Overview

  • Product name
    Human Ferritin (FTL) ELISA Kit
    See all Ferritin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 1.5 ng/ml
  • Range
    1.563 ng/ml - 50 ng/ml
  • Recovery

    97 %

  • Assay time
    4h 0m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Monkey
  • Product overview

    Abcam’s Ferritin (FTL) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ferritin concentrations in plasma, serum, milk, cerebrospinal fluid and cell culture supernatants.


    A Ferritin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ferritin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ferritin captured in plate.


    Get results in 90 minutes with Human Ferritin ELISA Kit (FLT) (ab200018) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 20ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Ferritin Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Ferritin Microplate (12 x 8 well strips) 1 unit
    Ferritin Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • Involvement in disease
    Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
    Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
  • Sequence similarities
    Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Information by UniProt
  • Alternative names
    • ferritin L chain
    • Ferritin L subunit
    • Ferritin light chain
    • ferritin light polypeptide
    • ferritin light polypeptide like 3
    • FRIL_HUMAN
    • FTL
    • NBIA3
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab108837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Human Ferritin measured in biological fluids showing quantity (pg) per mL of tested sample. Serum and plasma samples were diluted 10-20 fold. Milk, urine and PBMC supernatant were diluted 1-4 fold.

  • Human Ferritin standard curve: mean of duplicates (+/- SD)
  • Human Ferritin measured in biological fluids showing quantity (pg) per mL of tested sample.
  • Representative Standard Curve using ab108837

Protocols

References

This product has been referenced in:
See all 6 Publications for this product

Customer reviews and Q&As

1-6 of 6 Q&A

Answer

Thank you for contacting us.


The proper value in human plasma is 8-400 ng/ml. On average we get 60 ng/ml for our samples.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer



I can confirm that this kit is suitable for EDTA collected plasma.

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Question
Answer

One immunogen is a recombinant protein and the other one is a purified protein.

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Answer

Thank you very much for your reply. My colleague Jackie is out of the office today but I'll be happy to help you with this.

Do you need antibodies that are specific for ferritin heavy or ferritin light chains? Looking through our catalog, I only found one that has been tested in ELISA and that was raised against total ferritin, ab7332-

https://www.abcam.com/Ferritin-antibody-ab7332.html

Since this is a polyclonal raised against isolated nativeprotein, youcould potentially use it as the capture and detection antibody (if detection ab were conjugated). You could also use ab7332 as a detection antibody with a monoclonal capture antibody specific for heavy or light chain ferritin.

The only tested pair that we have for a ferritin protein is specific for mitochondrial ferritin, which I don't think is applicable to your studies but please let me know if this assumption is incorrect.

I am sorry that we don't have more of a selection, but please let me know if you have any other questions or need anything else from us.

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Answer

Thank you for contacting us. We have a kit that may be suitable for your requirements. Ferritin Human ELISA KIt (ab108837) is designed for detection of Human Ferritin in plasma, serum, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures Ferritin in less than 4 hours. More information can be found from the following link: www.abcam.com/ab108837 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. If you would like to make enquiries in Spanish please do, we have Spanish technical support specialists on our team.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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