Product nameHuman Fetuin A ELISA Kit (AHSG)
See all Alpha 2 HS Glycoprotein kits
Intra-assay Sample n Mean SD CV% Overall 5.1% Inter-assay Sample n Mean SD CV% Overall 7.1%
Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
Assay typeSandwich (quantitative)
Sensitivity> 3 ng/ml
Range3.13 ng/ml - 200 ng/ml
= 99 %
Assay time4h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Fetuin A (AHSG) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fetuin A concentrations in Human plasma, serum, urine, milk and cell culture supernatants.
A Fetuin A specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fetuin A specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fetuin A captured in plate.
Get higher sensitivity in only 90 minutes with Human Fetuin A ELISA Kit (AHSG) (ab185989) from our SimpleStep ELISA® range.
Tested applicationsSuitable for: Sandwich ELISAmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Fetuin A Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Fetuin A Microplate (12 x 8 well strips) 1 unit Fetuin A Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml
- Information by UniProt
SimpleStep ELISA kits
Our Abpromise guarantee covers the use of ab108855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
This product has been referenced in:
- Niu R et al. iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis. PLoS One 12:e0170741 (2017). ELISA ; Human . Read more (PubMed: 28122020) »
- Abdullah MI et al. Tissue and serum samples of patients with papillary thyroid cancer with and without benign background demonstrate different altered expression of proteins. PeerJ 4:e2450 (2016). Sandwich ELISA ; Human . Read more (PubMed: 27672505) »