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Cardiovascular Angiogenesis Growth Factors FGF FGF
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Human FGF-21 ELISA Kit (ab125966)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (4)

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Sandwich ELISA - FGF-21 Human ELISA Kit (ab125966)
  • Typical Standard Curve

Key features and details

  • Sensitivity: 0.03 ng/ml
  • Range: 0.0313 ng/ml - 2 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human FGF-21 ELISA Kit
    See all FGF21 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.03 ng/ml
  • Range

    0.031 ng/ml - 2 ng/ml
  • Recovery

    97 %

  • Assay time

    5h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s FGF-21 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human FGF-21 in plasma, serum, and cell culture supernatants.


    A FGF-21 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a FGF-21 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of FGF-21 captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    1X Standard Diluent 1 x 2ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human FGF-21 Antibody 1 x 120µl
    Chromogen Substrate 1 x 7ml
    FGF-21 Microplate (12 x 8 well strips) 1 unit
    FGF-21 Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 11ml
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • FGF
    • FGF
    • Signal Transduction
    • Growth Factors/Hormones
    • FGF
    • Cancer
    • Growth factors
    • FGF
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Growth factors and hormones ELISA kits
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Cellular metabolism
  • Function

    Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.
  • Sequence similarities

    Belongs to the heparin-binding growth factors family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q9NSA1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • FGF 21
    • FGF-21
    • Fgf21
    • FGF21_HUMAN
    • FGFL
    • Fibroblast growth factor 21
    • PRO10196
    • UNQ3115
    see all
  • Database links

    • Entrez Gene: 26291 Human
    • Omim: 609436 Human
    • SwissProt: Q9NSA1 Human
    • Unigene: 283015 Human

    Images

    • Sandwich ELISA - FGF-21 Human ELISA Kit (ab125966)
      Sandwich ELISA - FGF-21 Human ELISA Kit (ab125966)
      FGF-21 measured in biological fluids, background signal subtracted (duplicates +/- SD).
    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab125966

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab125966? Please let us know so that we can cite the reference in this datasheet.

    ab125966 has been referenced in 4 publications.

    • Peláez-Jaramillo MJ  et al. Impact of a Formulation Containing Unusual Polyunsaturated Fatty Acids, Trace Elements, Polyphenols and Plant Sterols on Insulin Resistance and Associated Disturbances. Diabetes Ther 11:229-245 (2020). PubMed: 31691133
    • Mölzer C  et al. Features of an altered AMPK metabolic pathway in Gilbert's Syndrome, and its role in metabolic health. Sci Rep 6:30051 (2016). Sandwich ELISA ; Human . PubMed: 27444220
    • Dekker Nitert M  et al. Placental fibroblast growth factor 21 is not altered in late-onset preeclampsia. Reprod Biol Endocrinol 13:14 (2015). Sandwich ELISA ; Human . PubMed: 25890271
    • Dekker Nitert M  et al. Increased placental expression of fibroblast growth factor 21 in gestational diabetes mellitus. J Clin Endocrinol Metab 99:E591-8 (2014). PubMed: 24432989

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