Overview

  • Product name

    Human FGF-21 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.03 ng/ml
  • Range

    0.031 ng/ml - 2 ng/ml
  • Recovery

    97 %

  • Assay time

    5h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s FGF-21 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human FGF-21 in plasma, serum, and cell culture supernatants.


    A FGF-21 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a FGF-21 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of FGF-21 captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab125966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • FGF-21 measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab125966

Protocols

References

This product has been referenced in:

  • Mölzer C  et al. Features of an altered AMPK metabolic pathway in Gilbert's Syndrome, and its role in metabolic health. Sci Rep 6:30051 (2016). Sandwich ELISA ; Human . Read more (PubMed: 27444220) »
  • Dekker Nitert M  et al. Placental fibroblast growth factor 21 is not altered in late-onset preeclampsia. Reprod Biol Endocrinol 13:14 (2015). Sandwich ELISA ; Human . Read more (PubMed: 25890271) »
See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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