Overview

  • Product name

    Human FGF basic ELISA Kit (FGF2)
    See all FGF basic kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma, Cell Lysate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 50 pg/ml
  • Range

    102.4 pg/ml - 10000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 93.48 83% - 103%
    Serum 95.46 84% - 104%
    Plasma 92.64 82% - 103%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Pig
  • Product overview

    Abcam’s FGF basic (FGF2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF basic in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human FGF basic coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF basic present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF basic antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF basic bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab99979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab99979.

  • Representative Standard Curve using ab99979.

Protocols

References

This product has been referenced in:

  • Zhang H  et al. MiR-148b-3p inhibits renal carcinoma cell growth and pro-angiogenic phenotype of endothelial cell potentially by modulating FGF2. Biomed Pharmacother 107:359-367 (2018). Read more (PubMed: 30099339) »
  • Ban MJ  et al. Fibroblast growth factor receptor 3-mediated reactivation of ERK signaling promotes head and neck squamous cancer cell insensitivity to MEK inhibition. Cancer Sci 109:3816-3825 (2018). Read more (PubMed: 30343534) »
See all 4 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Abreviews
To test if the two assays ab99979 and ab100670 are cross reactive I loaded the recombinant protein from one assay to the other and I got a very clear signal for mouse protein in the human ELISA and human protein in the mouse ELISA. The assays are not species specific and can't be use for xenograft samples to distinguish the source of FGF2.

Abcam user community

Verified customer

Submitted Jan 31 2017

Answer

Vielen Dank für Ihren Anruf.

Leider hat das Labornicht einpräzipitiertes FGF getestet in diesem ELISA, weder ein FGF präzipitiert mit Ammonium Sulfat oder Säure. Das Labor denkt auch, dass 10kD cut off (oder 5kD cut off) spin columns, die wahrscheinlich beste Methode wäre, um das FGF zu konzentrieren. Obschon nicht getestet, könnte es schon sein, dass das mitdem Ammoniumpräzipitat konzentrierte FGF im ELISA detektiert werden kann. Es sollte jedoch zuerst dialysiert werden, um das Salz von der Präzipitation herauszudialysieren.

Es tut mir leid, Ihnen keine definitivere Antwort geben zu können. Bitte zögern Sie nicht, uns wieder zu kontaktieren, sollten Sie weitere Fragen haben.

Read More

Answer

Thank you for contacting us with your enquiry, and for your patience while I have been in touch with the lab for more information.

We have not tested any of these kits in-house with cerebrospinal fluid, but the lab scientistsrecommend using ab99979 for testing CSF. To test this, we would suggest first running the CSF samples neat (undiluted) and diluting from there if needed. If dilution is necessary, make sure to use the Assay Diluent B.

We have a testing discount program that you may be interested in. For this program, you would purchase the FGF kit, test it with CSF, and submit data to us in the form of an Abreview. In exchange, we will give a discount worth the price of the kitapplied toa future purchase (within 4 months). Would you be interested in this program? I can send more information if you would like.

I hope this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

Read More

Answer

Thank you for contacting us.

These kits are provided with 12 strips of 8 wells each in a frame that is the same size as a standard 96-well plate (˜5 x 3.33"). If you need any more specific information, please let me know and I will be happy to help you further.

Read More

Answer

Thank you for contacting us.

This kit is suitable for FGF basic detection in serum, plasma, cell culture medium and urine.

The kit is sensitive to detect the FGFbasic as lower than 50pg/ml. If customer is interested in testing the level below 50 pg I would recommend the extending the standard dilution further 102.5, 40.96, 16.38 and 6.55 pg/ml

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up