• Product name
    Human FGF basic ELISA Kit (FGF2)
    See all FGF basic kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma, Cell Lysate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 50 pg/ml
  • Range
    102.4 pg/ml - 10000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 93.48 83% - 103%
    Serum 95.46 84% - 104%
    Plasma 92.64 82% - 103%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig
  • Product overview

    Abcam’s FGF basic (FGF2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF basic in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human FGF basic coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF basic present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF basic antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF basic bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform



Our Abpromise guarantee covers the use of ab99979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab99979.

  • Representative Standard Curve using ab99979.



This product has been referenced in:
  • Ding BS  et al. Endothelial MMP14 is required for endothelial-dependent growth support of human airway basal cells. J Cell Sci 128:2983-8 (2015). ELISA ; Human . Read more (PubMed: 26116571) »

See 1 Publication for this product

Customer reviews and Q&As

To test if the two assays ab99979 and ab100670 are cross reactive I loaded the recombinant protein from one assay to the other and I got a very clear signal for mouse protein in the human ELISA and human protein in the mouse ELISA. The assays are not species specific and can't be use for xenograft samples to distinguish the source of FGF2.

Abcam user community

Verified customer

Submitted Jan 31 2017

Vielen Dank für Ihren Anruf.

Leider hat das Labornicht einpräzipitiertes FGF getestet in diesem ELISA, weder ein FGF präzipitiert mit Ammonium Sulfat oder Säure. Das Labor denkt auch, dass 10kD cut off (oder 5kD cut off) s...

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Thank you for contacting us with your enquiry, and for your patience while I have been in touch with the lab for more information.

We have not tested any of these kits in-house with cerebrospinal fluid, but the lab scientistsrecommend using a...

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Thank you for contacting us.

These kits are provided with 12 strips of 8 wells each in a frame that is the same size as a standard 96-well plate (˜5 x 3.33"). If you need any more specific information, please let me know and I will be happy t...

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Thank you for contacting us.

This kit is suitable for FGF basic detection in serum, plasma, cell culture medium and urine.

The kit is sensitive to detect the FGFbasic as lower than 50pg/ml. If customer is interested in testing the ...

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