Overview

  • Product name

    Human FGF basic ELISA Kit (FGF2)
    See all FGF basic kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 50 pg/ml
  • Range

    102.4 pg/ml - 10000 pg/ml
  • Recovery

    > 95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 97.65 87% - 108%
    Tissue Extracts 95.87 86% - 107%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s FGF basic (FGF2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF basic in cell lysates and tissue lysates.

    This assay employs an antibody specific for Human FGF basic coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF basic present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF basic antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF basic bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab99980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • FGF2 measured in tissue lysates showing quantity (pg) per 1 mg of extracted protein. Protein concentration for samples varied from 7 mg/mL to 17 mg/mL. Samples were diluted 200-2000 fold.

  • FGF2 measured in cell culture lysates showing quantity (pg) per 1 mln cells. Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 200-2000 fold.

Protocols

References

This product has been referenced in:

  • Jaszberenyi M  et al. Potentiation of cytotoxic chemotherapy by growth hormone-releasing hormone agonists. Proc Natl Acad Sci U S A 111:781-6 (2014). Read more (PubMed: 24379381) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your inquiry.

Unfortunately, we have not tested the cross-reactivity for ab99980 with mouse sample. Thus, we do not have cross reactivity data for mouse sample with ab99980. Epitope mapping has also not been performed. At the moment, we know that ab99980 has been tested to react with bovine bFGF. Since human, mouse, and bovine bFGF share 96% homology, the chances of cross reactivity is expected to be fairly high.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

Both the primary antibody specific for human FGF and the biotinylated secondary antibody used in this kit are monoclonal.

Unfortunately we do not sell these antibodies separately however, we do sell anti-FGF antibodies which have been tested for use in sandwich ELISA in our catalogue.

For example Anti-FGF basic antibody [EP1735] (ab92337) (https://www.abcam.com/ab92337) which is a rabbit monoclonal, is compatible for use with the capture antibody mouse monoclonal [AS24] to FGF basic (ab17279) (https://www.abcam.com//ab17279) and is guaranteed for use in sandwich ELISA in human.

Alternatively, Anti-FGF basic antibody (Biotin) (ab84027) (https://www.abcam.com/ab84027) can be used with the capture antibody rabbit polyclonal to FGF basic (ab109613) (https://www.abcam.com/ab109613).

If you would like more help in choosing a suitable product, I would be more than happy to help.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you very much for contacting us.

Unfortunately, epitope mapping of the two antibody pairs has not been performed, so we do not know the exact amino acid sequence recognised and if these regions are specific for human and mouse, respectively. All we know for the antibody pair in the mouse FGF ELISA kit is that the antibody pair is binding on the whole mature protein between amino acids x and y.

Due to the high degree of conservation of basic FGF the mouse FGF ELISA Kit ab100670 will indeed also recognise human FGF2 and vice versa, as internal testing data also show.

I am sorry that I cannot be of more assistance on this occasion, and hope this information is nevertheless helpful to you. Should you have any further questions, please do not hesitate to contact us.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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