Overview

  • Product name

    Human FGF1 ELISA Kit
    See all FGF1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    extract 8 3.4%
    Inter-assay
    Sample n Mean SD CV%
    extract 3 3.8%
  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Tissue Extracts, Cell culture media
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.97 pg/ml
  • Range

    18.75 pg/ml - 1200 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 88 87% - 91%
    Urine 81 78% - 83%
    Serum 91 89% - 94%
    Tissue Extracts 103 101% - 104%
    Cell culture media 114 112% - 115%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Product overview

    FGF1 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of FGF1 protein in human serum, urine, saliva, cell culture supernatant, and tissue extract samples. 


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm. 


    Fibroblast growth factor 1 (FGF1) is a heparin-binding fibroblast growth factor that plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Specifically, FGF1 binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation. Endogenous FGF-1 is found in the nucleus of most cell types. Nuclear localization is required for FGF-1 mitogenic activity. FGF-1 promotes tumor development by promoting cancer cell proliferation and survival. Mouse and rat FGF1 are 95.5% identical to human FGF1.


     Sensitivity:
    Samples diluted in Sample Diluent NS – 4.42 pg/mL
    Samples diluted in Sample Diluent 50BS – 5.17 pg/mL
    Samples diluted in 1X Cell Extraction Buffer PTR – 1.97 pg/mL


     

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human FGF1 Capture Antibody 1 x 600µl
    10X Human FGF1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    Human FGF1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 50BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
  • Sequence similarities

    Belongs to the heparin-binding growth factors family.
  • Cellular localization

    Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
  • Information by UniProt
  • Alternative names

    • Acidic fibroblast growth factor
    • aFGF
    • Beta endothelial cell growth factor
    • Beta-endothelial cell growth factor
    • ECGF
    • ECGF beta
    • ECGF-beta
    • ECGFA
    • ECGFB
    • Endothelial Cell Growth Factor alpha
    • Endothelial Cell Growth Factor beta
    • FGF 1
    • FGF alpha
    • Fgf1
    • FGF1_HUMAN
    • FGFA
    • Fibroblast growth factor 1
    • Fibroblast Growth Factor 1 Acidic
    • GLIO703
    • HBGF 1
    • HBGF-1
    • HBGF1
    • Heparin binding growth factor 1
    • Heparin binding growth factor 1 precursor
    • Heparin-binding growth factor 1
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab219636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of FGF1 were measured in duplicates, interpolated from the FGF1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, urine 50%, saliva 50%, and stimulated PBMC media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF1 concentration was determined to be 868 pg/mL in neat serum, 1,275 pg/mL in neat urine, 903 pg/mL in neat saliva, and 518 pg/mL in neat stimulated PBMC culture media.

  • The concentrations of FGF1 were measured in duplicate and interpolated from the FGF1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF1 concentration was determined to be 886 pg/mL in human brain extract, 179 pg/mL in mouse brain extract, and 68 pg/mL in rat brain extract.

  • The concentrations of FGF1 were measured in duplicate and interpolated from the FGF1 standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean FGF1 concentration was determined to be 445 pg/mL in human brain extract, 161 pg/mL in mouse brain extract, and 61 pg/mL in rat brain extract.

  • The concentrations of FGF1 were measured in duplicates and interpolated from the FGF1 standard curves. Undiluted samples are as follows: unstimulated PBMC media 100% and stimulated PBMC media 100%. The interpolated values are plotted (mean +/- SD, n=2). The level of FGF1 in neat unstimulated PBMC media was below the 7th point of the standard curve and could not be interpolated. The mean FGF1 concentration was determined to be 27 pg/mL in neat stimulated PBMC media. PBMC media samples were cultured in RPMI media with 10% fetal bovine serum (unstimulated) for 24 hours and then stimulated for 48 hours with 1.5% PHAM.

Protocols

References

ab219636 has not yet been referenced specifically in any publications.

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