Overview

  • Product name

    Human FGF19 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Serum, Plasma, Cell culture media
  • Assay type

    Sandwich
  • Sensitivity

    30 pg/ml
  • Range

    30 pg/ml - 8000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 108.5 101% - 117%
    Plasma 107.4 97% - 118%
    Cell culture media 95.99 80% - 113%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam's FGF19 Human ELISA Kit (ab193703) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FGF19 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for human FGF19 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures FGF19 present in the samples. The wells are washed and biotinylated anti-human FGF19 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of FGF19 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent C 1 x 30ml
    Biotinylated Human FGF-19 detection antibody (lyophilized) 2 vials
    Human FGF-19 Standards (lyophilized) 2 vials
    Pre-coated Human FGF-19 Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Function

    Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4.
  • Tissue specificity

    Expressed in fetal brain, cartilage, retina, and adult gall bladder.
  • Sequence similarities

    Belongs to the heparin-binding growth factors family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • FGF-19
    • FGF19
    • FGF19_HUMAN
    • Fibroblast growth factor 19
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

Protocols

References

This product has been referenced in:

  • Dash A  et al. Pharmacotoxicology of clinically-relevant concentrations of obeticholic acid in an organotypic human hepatocyte system. Toxicol In Vitro 39:93-103 (2017). Read more (PubMed: 27939613) »
See 1 Publication for this product

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