Overview

  • Product name

    Human FGF21 ELISA Kit
    See all FGF21 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 5.3%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5.5%
  • Sample type

    Milk, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    3.3 pg/ml
  • Range

    31.25 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Milk 108 107% - 110%
    Serum 93 86% - 104%
    Heparin Plasma 91 90% - 93%
    EDTA Plasma 92 85% - 97%
    Citrate Plasma 74 72% - 77%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse
  • Product overview

    FGF-21 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human FGF-21 protein in serum, plasma, and milk.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    FGF-21, or Fibroblast growth factor 21, is a member of the fibroblast growth factor family encoded by the gene FGF21. FGF-21 helps regulate glucose homeostasis by mediating liver signaling to the brain by inducing phosphorylation of the mitogen-activated protein kinase extracellular signal-related kinase 1. FGF-21 is principally expressed in the liver in response to fasting, where it mediates several metabolic responses such as ketogenesis, hepatic fatty acid oxidation, and resistance to growth hormones. In addition, it is involved with embryonic development, cell growth, morphogenesis, and tissue repair. Serum levels of FGF-21 have been found to be elevated in patients with type 2 diabetes mellitus, and higher levels of FGF-21 also correlate with higher liver fat content.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human FGF-21 Capture Antibody 1 x 600µl
    10X Human FGF-21 Detector Antibody 1 x 600µl
    Human FGF-21 Lyophilized Recombinant Protein 2 vials
    Antibody Diluent 5BI 1 x 6ml
    10X Wash Buffer PT (ab206977) 1 x 20ml
    TMB Development Solution 1 x 12ml
    Stop Solution 1 x 12ml
    Sample Diluent NS 1 x 50ml
    Plate Seals 1 unit
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
  • Research areas

  • Function

    Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.
  • Sequence similarities

    Belongs to the heparin-binding growth factors family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • FGF 21
    • FGF-21
    • Fgf21
    • FGF21_HUMAN
    • FGFL
    • Fibroblast growth factor 21
    • PRO10196
    • UNQ3115
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab222506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed

  • The concentrations of FGF-21 were measured in duplicates, interpolated from the FGF-21 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (EDTA) 100%, and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF-21 concentration was determined to be 657 pg/mL in neat serum, 772 pg/mL in neat plasma (citrate), 289 pg/mL in neat plasma (EDTA), and 711 pg/mL in neat plasma (heparin).

  • The concentrations of FGF-21 was measured in duplicates, interpolated from the FGF-21 standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF-21 concentration was determined to be 413 pg/mL with a range of 59.4 – 1,537 pg/mL.

Protocols

References

ab222506 has not yet been referenced specifically in any publications.

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