Product nameHuman FGF4 ELISA Kit
See all FGF4 kits
Sample typeCell culture supernatant, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 50 pg/ml
Range24.69 pg/ml - 18000 pg/ml
> 90 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 91.54 80% - 103% Plasma 96.21 88% - 110%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s FGF4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF4 in plasma and cell culture supernatants. (Human FGF4 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human FGF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human FGF4 in normal serum with ab100516, only in serum spiked with Human FGF4.
Optimization may be required with urine samples.
Tested applicationsSuitable for: Sandwich ELISAmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 120X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human FGF4 2 units FGF4 Microplate (12 strips x 8 wells) 1 unit Recombinant Human FGF4 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceFGF4 is a heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF4 signals through the FGFR 1c, 2c, 3c, and 4.
- Fibroblast growth factor 4
- Heparin binding growth factor 4
- SwissProt: P08620 Human
Our Abpromise guarantee covers the use of ab100516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100516 has not yet been referenced specifically in any publications.