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|Sample type||Average %||Range|
|Cell culture supernatant||91.54||80% - 103%|
|Plasma||96.21||88% - 110%|
Abcam’s FGF4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF4 in plasma and cell culture supernatants. (Human FGF4 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human FGF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human FGF4 in normal serum with ab100516, only in serum spiked with Human FGF4.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|120X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Human FGF4||2 units|
|FGF4 Microplate (12 strips x 8 wells)||1 unit|
|Recombinant Human FGF4 Standard (lyophilized)||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100516 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"