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Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity (ab256605)

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Sandwich ELISA - Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity (ab256605)
  • Sandwich ELISA - Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity (ab256605)
  • Sandwich ELISA - Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity (ab256605)
  • Sandwich ELISA - Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity (ab256605)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

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Overview

  • Product name

    Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity
    See all Fibrinogen kits
  • Assay type

    ELISA set
  • Range

    125 pg/ml - 8000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human Fibrinogen High Sensitivity. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab241383), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human Fibrinogen Capture Antibody (unconjugated) 1 x 100µg
    Human Fibrinogen Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Blood
    • Coagulation
    • Common
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other
    • Metabolism
    • Types of disease
    • Metabolic disorders
  • Function

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.
  • Domain

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    modifications

    The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02671 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • FGA
    • FGB
    • FGG
    • Fib2
    • FIBA_HUMAN
    • Fibrinogen A alpha polypeptide
    • Fibrinogen alpha chain
    • Fibrinogen B alpha polypeptide
    • Fibrinogen beta chain
    • Fibrinogen G alpha polypeptide
    • Fibrinogen gamma chain
    • fibrinogen, B beta polypeptide
    • fibrinogen, G gamma polypeptide
    • fibrinogen, gamma polypeptide
    • Fibrinogen--alpha -polypeptide chain
    • Fibrinogen--beta -polypeptide chain
    • Fibrinogen--gamma-polypeptide chain
    see all
  • Database links

    • Entrez Gene: 2244 Human
    • Entrez Gene: 2266 Human
    • Entrez Gene: 2243 Human
    • Omim: 134820 Human
    • Omim: 134850 Human
    • Omim: 134830 Human
    • SwissProt: P02671 Human
    • SwissProt: P02675 Human
    • SwissProt: P02679 Human
    • Unigene: 351593 Human
    see all

Associated products

  • Alternative Versions

    • Human Fibrinogen ELISA Kit - high sensitivity (ab241383)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab256605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
Use at an assay dependent concentration.
Notes
Sandwich ELISA
Use at an assay dependent concentration.

Images

  • Sandwich ELISA - Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity (ab256605)
    Sandwich ELISA - Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity (ab256605)
    Representative standard curve from corresponding SimpleStep ELISA® Kit (ab241383), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
  • Sandwich ELISA - Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity (ab256605)
    Sandwich ELISA - Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity (ab256605)
    Representative standard curve from corresponding SimpleStep ELISA® Kit (ab241383), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
  • Sandwich ELISA - Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity (ab256605)
    Sandwich ELISA - Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity (ab256605)
    Representative standard curve from corresponding SimpleStep ELISA® Kit (ab241383), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
  • Sandwich ELISA - Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity (ab256605)
    Sandwich ELISA - Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity (ab256605)
    To learn more about the advantages of recombinant antibodies see here.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab256605? Please let us know so that we can cite the reference in this datasheet.

ab256605 has not yet been referenced specifically in any publications.

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