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Human Fibrinogen ELISA Kit (ab108841)

Reviews (1)Q&A (4)References (7)
Typical Standard Curve

    Key features and details

    • Sensitivity: 1 ng/ml
    • Range: 1.25 ng/ml - 80 ng/ml
    • Sample type: Cell culture supernatant, Milk, Saliva, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    Overview

    • Product name

      Human Fibrinogen ELISA Kit
      See all Fibrinogen kits

    • Detection method

      Colorimetric

    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall 4%
      Inter-assay
      Sample n Mean SD CV%
      Overall 8.6%

    • Sample type

      Cell culture supernatant, Saliva, Milk, Urine

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      = 1 ng/ml

    • Range

      1.25 ng/ml - 80 ng/ml

    • Recovery

      96 %

    • Assay time

      4h 00m

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Human

    • Product overview

      Human Fibrinogen ELISA kit is designed for the quantitative measurement of fibrinogen concentrations in cell culture media, saliva, milk and urine samples.


      A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibrinogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibrinogen captured in plate.


      Get better reproducibility in only 90 minutes with Human Fibrinogen ELISA Kit (ab208036) from our SimpleStep ELISA® range.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      100X Streptavidin-Peroxidase Conjugate 1 x 80µl
      10X Diluent N Concentrate 1 x 30ml
      20X Wash Buffer Concentrate 2 x 30ml
      50X Biotinylated Human Fibrinogen Antibody 1 x 120µl
      Chromogen Substrate 1 x 8ml
      Fibrinogen Microplate (12 x 8 well strips) 1 unit
      Fibrinogen Standard 1 vial
      Sealing Tapes 3 units
      Stop Solution 1 x 12ml

    • Research areas

    • Function

      Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

    • Tissue specificity

      Plasma.

    • Involvement in disease

      Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
      Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.

    • Sequence similarities

      Contains 1 fibrinogen C-terminal domain.

    • Domain

      A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.

    • Post-translational
      modifications

      The alpha chain is not glycosylated.
      Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
      About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
      Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
      Phosphorylation sites are present in the extracellular medium.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Alternative names

      • FGA
      • FGB
      • FGG
      • Fib2
      • FIBA_HUMAN
      • Fibrinogen A alpha polypeptide
      • Fibrinogen alpha chain
      • Fibrinogen B alpha polypeptide
      • Fibrinogen beta chain
      • Fibrinogen G alpha polypeptide
      • Fibrinogen gamma chain
      • fibrinogen, B beta polypeptide
      • fibrinogen, G gamma polypeptide
      • fibrinogen, gamma polypeptide
      • Fibrinogen--alpha -polypeptide chain
      • Fibrinogen--beta -polypeptide chain
      • Fibrinogen--gamma-polypeptide chain
      see all

    • Database links

      see all

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108841.

    References (7)

    Publishing research using ab108841? Please let us know so that we can cite the reference in this datasheet.

    ab108841 has been referenced in 7 publications.

    • Ahlfeld T  et al. Toward Biofabrication of Resorbable Implants Consisting of a Calcium Phosphate Cement and Fibrin-A Characterization In Vitro and In Vivo. Int J Mol Sci 22:N/A (2021). PubMed: 33530649
    • Mosleth EF  et al. Cerebrospinal fluid proteome shows disrupted neuronal development in multiple sclerosis. Sci Rep 11:4087 (2021). PubMed: 33602999
    • Chu HW  et al. Identification of Salivary Biomarkers for Oral Cancer Detection with Untargeted and Targeted Quantitative Proteomics Approaches. Mol Cell Proteomics 18:1796-1806 (2019). PubMed: 31253657
    • Zitur LJ  et al. Brain Death Enhances Activation of the Innate Immune System and Leads to Reduced Renal Metabolic Gene Expression. Transplantation 103:1821-1833 (2019). PubMed: 30964836
    • Zhang Y  et al. Application of Isobaric Tags for Relative and Absolute Quantification (iTRAQ) Coupled with Two-Dimensional Liquid Chromatography/Tandem Mass Spectrometry in Quantitative Proteomic Analysis for Discovery of Serum Biomarkers for Idiopathic Pulmonary Fibrosis. Med Sci Monit 24:4146-4153 (2018). PubMed: 29909421
    • Wang H  et al. Urinary Fibrinogen as a Predictor of Progression of CKD. Clin J Am Soc Nephrol 12:1922-1929 (2017). PubMed: 28903970
    • Hattori K  et al. Increased cerebrospinal fluid fibrinogen in major depressive disorder. Sci Rep 5:11412 (2015). PubMed: 26081315

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Cross reactive with Rhesus EDTA plasma samples

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    abreview image
    An experience laboratory technician completed this Fibrinogen ELISA from Abcam, catalog number AB108841, lot GR245646-10. The goal of the experiment was to determine the cross-reactivity of this kit for EDTA-prepared plasma samples and serum samples for M. mulatta. Samples were stored prior to thaw at -80 degrees Celsius.

    Overall, the kit, like other Abcam ELISA kits, was easy to follow and straight-forward. The laboratory technician had no problem understanding any parts of the protocol.

    Our test was performed in 2 basic steps. The first was to find the appropriate testable dilution in plasma and serum, and the second was to test the linearity and spike recovery.

    To this end our first experiment tested 8 dilutions of both serum and plasma ranging from 1:10 to 1:640 (8 1:2 serial dilutions). In these dilution ranges, the serum was well within the OD range, however no linearity was observed in any part of the dilution scheme. The EDTA plasma was beyond the range of detection (under-diluted).

    Our second experiment focused on the EDTA prepared plasma. We diluted n=2 rhesus plasma samples 1:10,000, 1:30,000, 1:90,000, and 1:270,000 and tested in duplicate (as were the standards). The rest of the assay was performed to protocol’s specifications. For both animals tested, 1:90,000 and 1:270,000 gave similar results, suggesting this is the linear range of the assay.

    Absorbance measurements of the diluted samples were comparable between duplicates in both tests (pipetting error appeared to be at a minimum). We also performed a spike recovery in both pure diluent and diluted plasma. ~100% of spiked human Fibrinogen was detected in the 90,000 and 270,000 diluted samples.

    Overall, we recommend this kit for detection of M. mulatta fibrinogen in EDTA-prepared plasma.

    Abcam user community

    Verified customer

    Submitted Jun 07 2016

    Question

    For ab108841: can plasma/serum samples be used? with EDTA as anti-coagulant?
    For ab171578, ab108842: can plasma/serum with EDTA as anti-coagulant be used with this kit?

    Read More
    Answer

    Plasma treated with EDTA anticoagulant can be used with all three of these ELISA kits. However, if choosing between ab108842 and ab108841, the competitive ELISA ab108842 is preferable to ab108841 for this sample type. The SimpleStep ELISA ab171578 is preferable to both for plasma samples, given the relative simplicity of the protocol and the data for plasma samples that is presented on the datasheet.

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    Question

    What volume should be used to reconstitute the standard of lot GR59910-11?

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    Answer



    For this lot, there are 400 ng of standard per vial. So, reconstitute in 5 mL of the diluent for a 80 ng/mL standard solution.

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    Question

    Dear,

    For My experiment, I need an ELISA for fibrinogen detection for mice and human for my some experiments. I just check on website that you have both type of ELISAs but i was not able to find the cross reactivity with opposite one. We will prefer an ELISA which may detect both human and mice fibrinogen (cross-reactive). Kindly provide me the information ASAP if available.

    Read More
    Answer

    Thank you for you for your enquiry and your interest in our products.

    I have conducted a search for you using our advanced search engine. Currently, we only have ELISA kits which are specific for either human (ab108841, ab108842) or mouse fibrinogen (ab108843, ab10844). Unfortunately, we do not have any kits which would recognize both species.

    Apologies!

    If you need any further assistance in the future, please do not hesitate to contact me.

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    Question

    To refine my choice, I would like to know against which protein(s) (sequence or accesion number in Swissprot) are exactly directed antibodies used in the following Elisa kits:   ab108884 ab108883 ab108823 ab108841   Thanks in advance      

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    Answer

    Thank you for your inquiry. ab108884 is an alpha 1 microglobulin human ELISA kit, Swiss Prot P02760. Alpha1-microglobulin (1M), also called protein HC, is a tubular plasma and tissue protein that belongs to the lipocalin transport protein superfamily for small hydrophobic molecules. It contains 184 amino acids and weighs 26-kDa (1-2). Mature 1M and bikunin (urinary trypsin inhibitor) result from a common precursor (3). (1) Grubb AO et al. (1983) Biol Chem. 258:14698-14707 (2) Ekström B and Berggård I. (1977) J Biol Chem 252:8048-8057 (3) Vetr H and Gebhard W (1990) Biol Chem Hoppe Seyler. 371:1185-1196 https://www.abcam.com/alpha-1-Microglobulin-Human-ELISA-Kit-ab108884.html http://www.uniprot.org/uniprot/P02760#PRO_0000017886 ab108883 is an is an alpha 2 macroglobulin human ELISA kit, Swiss Prot P01023. It's a major serum protein. (1) Pineda-Salgado L et al (2005) Gene Expr Patterns. 6(1):3-10 (2) Jensen PE et al (2004) Biocim Biophys Acta. 5; 1690(3): 203-7 (3) Yang AH et al (1997) Nephrol Dial Transplant 12(3): 465-9 (4) Shiota G et al (1995) J Med 26(5-6): 295-308. https://www.abcam.com/alpha-2-Macroglobulin-Human-ELISA-Kit-ab108883.html http://www.uniprot.org/uniprot/P01023 ab108823 is a complement C3 human ELISA kit, Swiss Prot P01024.  Complement protein C3 is the fourth component to attach in the complement reaction sequence. It is a beta-globulin with a sedimentation coefficient of 5.5 and a molecular weight of 185,000. (1) Sahu A et al. (2001) Immunol. Rev. 180: 35-48 (2) Sacks S et al. 92003) J Mol Med. 81(7): 404-10 (3) Ramos-Casals M et al. (2004) Rheumatology (Oxford). (4) Gardinali M et al. (1998) Clin.Immunol.Immunopathol .87(3): 97-303 https://www.abcam.com/Complement-C3-Human-ELISA-kit-ab108823.html http://www.uniprot.org/uniprot/P01024 ab108841 is a fibrinogen human ELISA kit, Swiss Prot P02671, P02675, and P02679.  Fibrinogen (FBG) is a homodimer of molecular mass 340 kDa, made up of two sets of α, β, γ polypeptide chains, and synthesized in the parenchymal cell of the hepatocyte and in the megakaryocyte (1). FBG plays a major role in coagulation, and both elevated and decreased levels have clinical significance. Upon cleavage by thrombin in the initial stages of coagulation activation, FBG self-assembles to yield a fibrin clot matrix that subsequently is crosslinked by factor XIIIa to form an insoluble network. FBG also binds to the platelet glycoprotein IIbIIIa receptor so as to form bridges between platelets, thus facilitating aggregation (2). Elevated plasma FBG has been identified as an independent risk factor for coronary atherosclerosis and ischemic heart disease (3, 4). Individuals with congenital absence of FBG, termed afibrinogenemia, have prolonged bleeding times. (1) Doolittle, R.F. (1984) Annu. Rev. Biochem 53:195 (2) Handley, D.A. and Hughes, T.E. (1997) Thromb. Res. 87:1 (3) Handa, K. et al. (1989) Atherosclerosis 77:209 (4) Mannucci, P.M. and Mari, D. (1993) Fibrinolysis 3:51 (5) Amiral J. (1995) Clin. Appl. Thrombosis Hemostasis 1:243 http://www.uniprot.org/uniprot/P02671 http://www.uniprot.org/uniprot/P02675 http://www.uniprot.org/uniprot/P02679 I hope this information helps.  Please contact us with any other questions.

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