Human Fibrinogen ELISA Kit (ab108842)

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Overview

  • Product name

    Human Fibrinogen ELISA Kit
    See all Fibrinogen kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.5%

  • Sample type

    Plasma

  • Assay type

    Competitive

  • Sensitivity

    = 0.16 µg/ml

  • Range

    0.165 µg/ml - 40 µg/ml

  • Recovery

    97 %

  • Assay time

    3h 00m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Fibrinogen in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibrinogen levels in plasma.


    A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Fibrinogen is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Fibrinogen captured in plate.


    Get better reproducibility in only 90 minutes with Human Fibrinogen ELISA Kit (ab208036) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    1X Biotinylated Human Fibrinogen (Lyophilized) 1 vial
    20X Wash Buffer Concentrate 1 x 30ml
    Chromogen Substrate 1 x 8ml
    Fibrinogen Microplate (12 x 8 well strips) 1 unit
    Fibrinogen Standard 2 vials
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml

  • Research areas

  • Function

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

  • Tissue specificity

    Plasma.

  • Involvement in disease

    Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.

  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.

  • Domain

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.

  • Post-translational
    modifications

    The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracellular medium.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • FGA
    • FGB
    • FGG
    • Fib2
    • FIBA_HUMAN
    • Fibrinogen A alpha polypeptide
    • Fibrinogen alpha chain
    • Fibrinogen B alpha polypeptide
    • Fibrinogen beta chain
    • Fibrinogen G alpha polypeptide
    • Fibrinogen gamma chain
    • fibrinogen, B beta polypeptide
    • fibrinogen, G gamma polypeptide
    • fibrinogen, gamma polypeptide
    • Fibrinogen--alpha -polypeptide chain
    • Fibrinogen--beta -polypeptide chain
    • Fibrinogen--gamma-polypeptide chain
    see all

  • Database links

    see all

Associated products

Images

  • Sandwich ELISA - ab108842 Fibrinogen Human ELISA Kit
    Sandwich ELISA - ab108842 Fibrinogen Human ELISA Kit

    Fibrinogen measured in various samples showing quantity (micrograms) per mL of tested sample

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab108842.

Protocols

References

This product has been referenced in:

  • Liang Y  et al. Coagulation cascade and complement system in systemic lupus erythematosus. Oncotarget 9:14862-14881 (2018). Read more (PubMed: 29599912) »
  • Campbell MD  et al. An additional bolus of rapid-acting insulin to normalise postprandial cardiovascular risk factors following a high-carbohydrate high-fat meal in patients with type 1 diabetes: A randomised controlled trial. Diab Vasc Dis Res 14:336-344 (2017). Read more (PubMed: 28322071) »
See all 3 Publications for this product

Publishing research using ab108842? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

For ab108841: can plasma/serum samples be used? with EDTA as anti-coagulant?
For ab171578, ab108842: can plasma/serum with EDTA as anti-coagulant be used with this kit?

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Answer

Plasma treated with EDTA anticoagulant can be used with all three of these ELISA kits. However, if choosing between ab108842 and ab108841, the competitive ELISA ab108842 is preferable to ab108841 for this sample type. The SimpleStep ELISA ab171578 is preferable to both for plasma samples, given the relative simplicity of the protocol and the data for plasma samples that is presented on the datasheet.

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Question

So, this means my data is usable??

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Answer

Thank you for your reply.

Yes! Competitive ELISAs are a little different. Here is the background.

Competitive ELISA: Basic Principles

The central event of competitive ELISA is a competitive binding process executed by original antigen (sample antigen) and add-in antigen. The procedures of competitive ELISA are different in some respects compared with Indirect ELISA, Sandwich ELISA and Direct ELISA. A simplized procedure list is as follow:

Primary antibody (unlabeled) is incubated with sample antigen.
Antibody-antigen complexes are then added to 96-well plates which are pre-coated with the same antigen.
Unbound antibody is removed by washing the plate. (The more antigen in the sample, the less antibody will be able to bind to the antigen in the well, hence "competition.")
The secondary antibody that is specific to the primary antibody and conjugated with an enzyme is added.
A substrate is added, and remaining enzymes elicit a chromogenic or fluorescent signal.



For competitive ELISA, the higher the sample antigen concentration, the weaker the eventual signal. The major advantage of a competitive ELISA is the ability to use crude or impure samples and still selectively bind any antigen that may be present.

I hope this helps!

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Question

Dear,

For My experiment, I need an ELISA for fibrinogen detection for mice and human for my some experiments. I just check on website that you have both type of ELISAs but i was not able to find the cross reactivity with opposite one. We will prefer an ELISA which may detect both human and mice fibrinogen (cross-reactive). Kindly provide me the information ASAP if available.

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Answer

Thank you for you for your enquiry and your interest in our products.

I have conducted a search for you using our advanced search engine. Currently, we only have ELISA kits which are specific for either human (ab108841, ab108842) or mouse fibrinogen (ab108843, ab10844). Unfortunately, we do not have any kits which would recognize both species.

Apologies!

If you need any further assistance in the future, please do not hesitate to contact me.

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