Overview

  • Product name

    Human Fibronectin ELISA Kit
    See all Fibronectin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8%
  • Sample type

    Serum, Plasma
  • Assay type

    Competitive
  • Sensitivity

    = 0.7 µg/ml
  • Range

    0.78 µg/ml - 50 µg/ml
  • Recovery

    98 %

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Fibronectin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibronectin levels in plasma and serum.


    A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Fibronectin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Fibronectin captured in plate.


    Get results in 90 minutes with Human Fibronectin ELISA Kit (ab181419) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    1X Biotinylated Human Fibronectin (Lyophilized) 1 vial
    20X Wash Buffer Concentrate 1 x 30ml
    Chromogen Substrate 1 x 8ml
    Fibronectin Microplate (12 x 8 well strips) 1 unit
    Fibronectin Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificity

    Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in disease

    Glomerulopathy with fibronectin deposits 2
  • Sequence similarities

    Contains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stage

    Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    modifications

    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Alternative names

    • CIG
    • Cold insoluble globulin
    • Cold-insoluble globulin
    • DKFZp686F10164
    • DKFZp686H0342
    • DKFZp686I1370
    • DKFZp686O13149
    • ED B
    • Fibronectin 1
    • FINC
    • FINC_HUMAN
    • FN
    • FN1
    • FNZ
    • GFND
    • GFND2
    • LETS
    • Migration stimulating factor
    • MSF
    • Ugl-Y3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Fibronectin measured in various samples showing quantity (microgram) per mL of tested sample

  • Representative Standard Curve using ab108848.

Protocols

References

This product has been referenced in:

  • Mikula-Pietrasik J & Ksiazek K L-Carnosine Prevents the Pro-cancerogenic Activity of Senescent Peritoneal Mesothelium Towards Ovarian Cancer Cells. Anticancer Res 36:665-71 (2016). Read more (PubMed: 26851022) »
See 1 Publication for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Very easy ELISA, just a few hours and you'll have data

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Used citrate plasma samples which were diluted 1:100 as suggested in the protocol which I followed from start to finish.
Standard curve not quite straight in the higher concentrations, so appropriate sample dilution is important.

Very easy to use kit. Will buy it again.

Abcam user community

Verified customer

Submitted Oct 28 2014

Answer



I can confirm that this kit theoretically recognizes both form of fibronectin.

The insoluble cellular form will only be bound once by the antibody since they are monoclonal. Since fibronectin is then assembled into an insoluble matrix a single binding of antibody will underestimate the actual amount of insoluble cellular fibronectin.

We do not recommend this kit for insoluble cellular fibronectin.

ab108848 is tested and guaranteed for serum and plasma samples where cellular fibronectin does not interfere since both plasma and serum are cell free.

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Abreview for Fibronectin Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Serum (Human plasma)
Specification: Human plasma
Amount of Sample: 2.5 µg
Type of Kit Used: ELISA
Positive control: recombinant fibronectin
Did you use the Abcam Kit protocol?: Yes

Abcam user community

Verified customer

Submitted Jan 15 2013

Answer

Thank you very much for your interest our products.

To our knowledge, ab108848 has not been tested in urine. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

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Answer

Siento no haber contestado antes, me temo que no tengo la información de todos los productos solicitados, sin embargo, puedo proporcionarte acerca de los siguientes kits:

El kit PLTP Activity Fluorometric Assay Kit (ab65386) no es apropiado para vuestro propósito puesto que reacciona con el ortólogo murino.

Para LIF Human ELISA Kit (ab100582) no se han llevado a cabo tests de reactividad cruzada, por lo que se desconoce si reconoce la proteína murina. El anticuerpo de captura es monoclonal y el de detección policlonal. Ambas proteínas comparten un 78% de homología, por lo que algo de reactividad cruzada es de esperar.

Respecto a Complement C3 Human ELISA kit y Fibronectin Human ELISA Kit:

ab108823: se ha observado una reactividad cruzada del 0.5% con ratón y del 10% con rata.

ab108822: ninguna reactividad cruzada observada.

ab108847: no se ha observado reactividad cruzada con el ortólogo murino

ab108848: reactividad del 5% con ratón y del 20% con rata.

Con Fibronectin Human ELISA kit (with color giving dyes) (ab119524) se ha encontrado cierta reactividad cruzada de acuerdo con el colaborador que nos lo suministra.

Para ab119611 y ab119612 (uPA Human ELISA kit y uPA Receptor Human ELISA Kit) no se ha encontrado reactividad con la proteína de ratón.

No tengo de momento información acerca de ab133037 pero si sigues interesado, en cuanto la tenga te contacto de nuevo.

Si necesitáis un presupuesto o más información acerca de cualquiera de las opciones ofrecidas, por favor, no dudéis en contactarnos de nuevo y estaré encantada de poder ayudar.

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Answer

Thank you for your query.

I assume you customer 's kit has the lot number 1211713 (GR68088-1)?

I can confirm that the standard is HumanFibronectinin a buffered protein base (250 mg, lyophilized).

It should be reconstitutedwith + 2.5 mL = 100 ug/mL; Standard Curve pt 1 is a 2x dilution = 50 ug/ml.

I hope this information is helpful. Please do not hesitate to contact my withany further questions.

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