Overview

  • Product name

    Human Ficolin-2 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 4.78ng/ml 0.186 3.9%
    2 16 12.7ng/ml 0.648 5.1%
    3 16 24.1ng/ml 1.11 4.6%
    Inter-assay
    Sample n Mean SD CV%
    1 24 5.14ng/ml 0.298 5.8%
    2 24 13.2ng/ml 0.99 7.5%
    4 24 25.7ng/ml 1.62 6.3%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 20 pg/ml
  • Range

    0.78 ng/ml - 50 ng/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human Ficolin-2 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213778) is designed for the quantitative measurement of Human Ficolin-2 in cell culture supernatants, serum and plasma (heparin). 


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Ficolin-2 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: L26-A313) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ficolin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Ficolin-2 amount of sample captured in plate.

  • Notes

    Ficolin-2, which was initially identified as L-ficolin, is a protein that in humans is encoded by the FCN2 gene. The product of this gene belongs to the ficolin family of proteins. This family is characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. It is mapped to 9q34.3. This gene is predominantly expressed in the liver, and has been shown to have carbohydrate binding and opsonic activities. Serum concentration of ficolin-2 varies considerably in healthy individuals. FCN2 promoter polymorphisms were associated with marked changes in the ficolin-2 serum concentration.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human Ficolin-2 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human Ficolin-2 antibody 1 x 130µl
    Lyophilized recombinant Human Ficolin-2 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Sequence similarities

    Belongs to the ficolin lectin family.
    Contains 1 collagen-like domain.
    Contains 1 fibrinogen C-terminal domain.
  • Domain

    The fibrinogen-like domain (FBG) contains potential calcium-binding sites that may be involved in carbohydrate binding.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • 37 kDa elastin-binding protein
    • Collagen/fibrinogen domain containing protein 2
    • Collagen/fibrinogen domain-containing protein 2
    • EBP 37
    • EBP-37
    • EBP37
    • FCN 2
    • Fcn2
    • FCN2_HUMAN
    • FCNL
    • Ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)
    • Ficolin B
    • Ficolin beta
    • Ficolin-2
    • Ficolin-B
    • Ficolin-beta
    • Ficolin2
    • Hucolin
    • L ficolin
    • L-ficolin
    • OTTHUMP00000022518
    • P35
    • RP11 263F14.2
    • Serum lectin p35
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human Ficolin-2 ELISA Kit (ab213778) Standard Curve.

Protocols

References

ab213778 has not yet been referenced specifically in any publications.

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