Overview

  • Product name

    Human Ficolin-3 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 4.88ng/ml 0.215 4.4%
    2 16 12.6ng/ml 0.466 3.7%
    3 16 25.5ng/ml 1.17 4.6%
    Inter-assay
    Sample n Mean SD CV%
    1 24 5.14ng/ml 0.35 6.8%
    2 24 13.6ng/ml 1.02 7.5%
    3 24 25.7ng/ml 1.98 7.7%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 20 pg/ml
  • Range

    0.78 ng/ml - 50 ng/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human Ficolin-3 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213779) is designed for the quantitative measurement of Human Ficolin-3 in cell culture supernatants, serum and plasma (heparin).


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Ficolin-3 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: Q24-R299) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ficolin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Ficolin-3 amount of sample captured in plate.

  • Notes

    Ficolin-3, also known as HAKA1, is a protein that in humans is encoded by the FCN3 gene. It is mapped to 1p36.11. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Ficolin-3 is expressed as a 35-kD protein that reacts with systemic lupus erythematosus sera. Ficolin-3 does not bind fibronectin (FN1), elastin (ELN), or zymosan, and the lectin activity of the Ficolin-3 is calcium independent. Homozygosity for the variant in the FCN3 gene results in a recessive complement deficiency syndrome.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human Ficolin-3 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Human Ficolin-3 antibody 1 x 130µl
    Lyophilized recombinant Human Ficolin-3 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Relevance

    FCN3 (Ficolin 3) is involved in the serum exerting lectin activity. In human serum, there are two types of ficolins, both of which have lectin activity. FCN3 is a thermolabile beta 2 macroglycoprotein found in all human serum and is a member of the ficolin/opsonin p35 lectin family. It can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway.
  • Cellular localization

    Secreted. Note: Found in blood plasma, bronchus, alveolus and bile duct.
  • Alternative names

    • Collagen/fibrinogen domain containing lectin 3 p35
    • Collagen/fibrinogen domain containing protein 3
    • FCN 3
    • FCNH
    • Ficolin (collagen/fibrinogen domain containing) 3
    • Ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen)
    • Ficolin 3
    • H ficolin
    • HAKA1
    • Hakata antigen
    • MGC22543
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human Ficolin-3 ELISA Kit (ab213779) Standard Curve.

Protocols

References

ab213779 has not yet been referenced specifically in any publications.

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