Human Folate Binding Protein / FBP ELISA Kit (ab213781)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 46.9 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Folate Binding Protein / FBP ELISA Kit
See all Folate Binding Protein/FBP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 103pg/ml 7.41 7.2% 2 16 333pg/ml 22.31 6.7% 3 16 1306pg/ml 88.8 6.8% Inter-assay Sample n Mean SD CV% 1 24 108pg/ml 8.53 7.9% 2 24 312pg/ml 24.96 8% 3 24 1388pg/ml 119.36 8.6% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
46.9 pg/ml - 3000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human Folate Binding Protein / FBP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213781) is designed for the quantitative measurement of Human Folate Binding Protein / FBP in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and Human milk.
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Folate Binding Protein / FBP has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: R25-S234) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Folate Binding Protein / FBP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Folate Binding Protein / FBP amount of sample captured in plate.
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Notes
Folate Binding Protein / FBP adult is a protein that in humans is encoded by the Folate Binding Protein / FBP gene. The protein encoded by this gene is a member of the folate receptor (FOLR) family. It is mapped to 11q13.4. Folate Binding Protein / FBP expression in Jurkat cells facilitated MBG or EBO entry, and FR-blocking reagents inhibited infection of MBG or EBO. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. The Folate Binding Protein / FBP gene encodes the adult folate receptor, or folate-binding protein (FBP), which has a high affinity for folic acid and for several reduced folic acid derivatives, and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Folate Binding Protein / FBP is also an important regulator of milk protein synthesis.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human Folate Binding Protein / FBP coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Human Folate Binding Protein / FBP antibody 1 x 100µl Lyophilized recombinant Human Folate Binding Protein / FBP standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. -
Tissue specificity
Exclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. -
Involvement in disease
Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function. -
Sequence similarities
Belongs to the folate receptor family. -
Post-translational
modificationsEight disulfide bonds are present.
The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease. -
Cellular localization
Cell membrane. Secreted. - Information by UniProt
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Alternative names
- adult
- Adult folate binding protein
- Adult folate-binding protein
see all -
Database links
- Entrez Gene: 2348 Human
- Omim: 136430 Human
- SwissProt: P15328 Human
- Unigene: 73769 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab213781 has not yet been referenced specifically in any publications.