Overview

  • Product name

    Human Folate Binding Protein / FBP ELISA Kit
    See all Folate Binding Protein/FBP kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 388pg/ml 14.74 3.8%
    2 16 1124pg/ml 30.35 2.7%
    3 16 2015pg/ml 64.48 3.2%
    Inter-assay
    Sample n Mean SD CV%
    1 24 379pg/ml 24.26 6.4%
    2 24 1225pg/ml 91.88 7.5%
    3 24 1996pg/ml 153.7 7.7%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    46.9 pg/ml - 3000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human Folate Binding Protein / FBP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213781) is designed for the quantitative measurement of Human Folate Binding Protein / FBP in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and Human milk. 


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Folate Binding Protein / FBP has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: R25-S234) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Folate Binding Protein / FBP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Folate Binding Protein / FBP amount of sample captured in plate.

  • Notes

    Folate Binding Protein / FBP adult is a protein that in humans is encoded by the Folate Binding Protein / FBP gene. The protein encoded by this gene is a member of the folate receptor (FOLR) family. It is mapped to 11q13.4. Folate Binding Protein / FBP expression in Jurkat cells facilitated MBG or EBO entry, and FR-blocking reagents inhibited infection of MBG or EBO. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. The Folate Binding Protein / FBP gene encodes the adult folate receptor, or folate-binding protein (FBP), which has a high affinity for folic acid and for several reduced folic acid derivatives, and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Folate Binding Protein / FBP is also an important regulator of milk protein synthesis.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human Folate Binding Protein / FBP coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Human Folate Binding Protein / FBP antibody 1 x 130µl
    Lyophilized recombinant Human Folate Binding Protein / FBP standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells.
  • Tissue specificity

    Exclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum.
  • Involvement in disease

    Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function.
  • Sequence similarities

    Belongs to the folate receptor family.
  • Post-translational
    modifications

    Eight disulfide bonds are present.
    The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease.
  • Cellular localization

    Cell membrane. Secreted.
  • Information by UniProt
  • Alternative names

    • adult
    • Adult folate binding protein
    • Adult folate-binding protein
    • FBP
    • Folate Binding Protein
    • Folate receptor
    • Folate receptor 1
    • Folate Receptor 1 Adult
    • Folate Receptor 1 Precursor
    • Folate receptor adult
    • Folate receptor alpha
    • FOLR
    • FOLR1
    • FOLR1_HUMAN
    • FR alpha
    • FR-alpha
    • FRalpha
    • KB cells FBP
    • MOV18
    • Ovarian cancer associated antigen
    • Ovarian tumor associated antigen
    • Ovarian tumor associated antigen MOv18
    • Ovarian tumor-associated antigen MOv18
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human Folate Binding Protein / FBP ELISA Kit (ab213781) Standard Curve.

Protocols

References

ab213781 has not yet been referenced specifically in any publications.

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