Overview

  • Product name

    Human Follicle Stimulating Hormone ELISA Kit
    See all Follicle Stimulating Hormone kits
  • Detection method

    Colorimetric
  • Sample type

    Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 2.5 mIU/ml
  • Range

    2.5 mIU/ml - 200 mIU/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Follicle Stimulating Hormone in vitroELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Follicle Stimulating Hormone in Human serum.

    A 96-well plate has been precoated with anti-Follicle Stimulating Hormone antibodies. Samples and standards and are added to the wells, where Follicle Stimulating Hormone in the sample and standards binds to the precoated antibody. After incubation and washing, added Anti-Follicle Stimulating Hormone HRP conjugate binds to the antibody-Follicle Stimulating Hormone complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Follicle Stimulating Hormone in the sample and the intensity is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab108641

Protocols

References

This product has been referenced in:

  • Zheng R  et al. A novel PNPLA6 compound heterozygous mutation identified in a Chinese patient with Boucher-Neuhäuser syndrome. Mol Med Rep 18:261-267 (2018). Read more (PubMed: 29749493) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

I have been informed by the lab that while these are specific to human FSH, the mouse serum may cause some background. We recommend optimizing the tests by running a serial dilution of the mouse serum to determine which dilution factor gives the least background.

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Answer

Thank you for contacting us and your interest in our products.

TheFollicle Stimulating Hormone Human ELISA Kit (ab108641) uses and anti-alpha-FSH monoclonal antibody for the capture and an anti-beta-FSH monoclonal antibody for the detection. TheFSH kit therefore detects the whole FSH molecule, not the free monomers.

I hope this informationhas beenhelpful. If you have any further questions, please do not hesitate to contact us again.

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Answer

Thank you very much for your inquiry.

We do have no ELISA kit against LH, FSH or Progesterone which are tested against the rat proteins. We do have however such kits tested against the human protein, for which we have however not yet tested whether they can work with the rat protein.

I am inquiring with the laboratory whether we have any anti rat ELISA kits for these proteins which we could put in ourcatalogue and I will get back to you as soon as I know more.

Thank you for your patience. Please let me know if you have any further questions.

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