Associated products


Our Abpromise guarantee covers the use of ab23074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-FOXA1 antibody (ab5089)

  • Form

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

General Info

  • Alternative names

    • forkhead box A1
    • Forkhead box protein A1
    • FOX A1
    • FOXA1
    • hepatocyte nuclear factor 3 alpha
    • Hepatocyte nuclear factor 3-alpha
    • HNF 3A
    • HNF-3-alpha
    • HNF-3A
    • HNF3A
    • MGC33105
    • TCF 3A
    • TCF-3A
    • TCF3A
    • Transcription factor 3A
    see all
  • Function

    Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
  • Tissue specificity

    Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas.
  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.
  • Cellular localization

  • Information by UniProt


ab23074 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


I am very sorry to hear you are disappointed after using the FOXA1 peptide (aa 463-473, ab23074) with the Anti-FOXA1 antibody (ab55178).

The antibody is monoclonal antibody that will only bind one epitope in the FOXA1 protein. This antibody was generated with an immunogen corresponding to the amino acids 367-473.

Since this antibody give a very strong signal with endogenous positive control that was used, Ican explain the lack of signal with the peptide:

The immunogen is much longer than the peptide. And the antibody therefore binds somewhere between amino acid 376-463.

Unfortunately, we do not offer the immunogen peptide for ab55178 at the moment in our catalogue.I suggest to use the full length protein (ab98301, )as control if needed. The cell line that was used is also a good control.

I hope this information is helpful. Please do not hesitate to contact me again with any furtherquestions.

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