• Nature
  • Amino Acid Sequence
    • Species


Our Abpromise guarantee covers the use of ab16809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-FOXP3 antibody [mAbcam 450] (ab450), Anti-FOXP3 antibody (ab10901), Anti-FOXP3 antibody [mAbcam 22510] (ab22510), Anti-FOXP3 antibody [mAbcam 22510] (HRP) (ab22831)

  • Form
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • AIID
    • DIETER
    • Forkhead box P3
    • Forkhead box protein P3
    • FOXP3
    • FOXP3delta7
    • Immune dysregulation polyendocrinopathy enteropathy X linked
    • Immunodeficiency polyendocrinopathy enteropathy X linked
    • IPEX
    • JM2
    • MGC141961
    • MGC141963
    • OTTHUMP00000025832
    • OTTHUMP00000025833
    • OTTHUMP00000226737
    • PIDX
    • Scurfin
    • XPID
    see all
  • Function
    Probable transcription factor. Plays a critical role in the control of immune response.
  • Involvement in disease
    Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
  • Sequence similarities
    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • Cellular localization
  • Information by UniProt


  • Western blot using ab10901 at 1/500.

    Lane 1: HEK 293 lysate.
    Lane 2: HEK 293 lysate over expressing human FOXP3. 
    Lane 3: HEK 293 lysate over expressing mouse FOXP3.
    Lane 4: HEK 293 lysate with FOXP3 peptide ab16809.
    Lane 5: HEK 293 lysate over expressing human FOXP3 with FOXP3 peptide ab16809.
    Lane 6: HEK 293 lysate over expressing mouse FOXP3 with FOXP3 peptide ab16809.

    All lanes with ab10901.
    A no primary control lane showed no bands.
    Peptide at 1µg/ml.
    Secondary antibody: Goat polyclonal to Rabbit IgG (HRP) ab6721 1/5000.
    Exposure time: 1 minute.
    Expected molecular weight: 47kD
    Lysates at 20µg/lane.

    A no primary control lane showed no bands.


This product has been referenced in:
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