Human Fractalkine ELISA Kit (CX3CL1) (ab100522)
- Datasheet
- References
- Protocols
Overview
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Product name
Human Fractalkine ELISA Kit (CX3CL1)
See all CX3CL1 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 300 pg/ml -
Range
0.25 ng/ml - 60 ng/ml -
Recovery
93 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 94.47 83% - 102% Plasma 93.54 83% - 103% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s CX3CL1 (Fraktaline) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CX3CL1 in plasma and cell culture supernatants. (Human CX3CL1 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for human CX3CL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CX3CL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CX3CL1 antibody is added. After washing away unbound biotinylated antibody, HRPconjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CX3CL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human Fractalkine in normal serum with ab100522, only in serum spiked with Human Fractalkine.
Get higher sensitivity in only 90 minutes with Human Fractalkine ELISA Kit (CX3Cl1) (ab192145) from our SimpleStep ELISA® range.
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Notes
Optimization may be required with urine samples
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 320X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CX3CL1 2 vials CX3CL1 Microplate (12 x 8 well strips) 1 unit Recombinant Human CX3CL1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion. -
Alternative names
- A 152E5.2
- AB030188
- ABCD 3
see all -
Database links
- Entrez Gene: 6376 Human
- Omim: 601880 Human
- SwissProt: P78423 Human
Associated products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab100522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Typical Standard CurveRepresentative Standard Curve using ab100522.
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Typical Standard CurveRepresentative Standard Curve using ab100522.
Protocols
References
ab100522 has not yet been referenced specifically in any publications.
