Overview

  • Product name

    Human Free Estriol ELISA Kit
    See all Estriol kits
  • Detection method

    Colorimetric
  • Sample type

    Serum, Hep Plasma, Cit plasma
  • Assay type

    Competitive
  • Sensitivity

    = 0.05 ng/ml
  • Range

    0.1 ng/ml - 12 ng/ml
  • Recovery

    101.6 %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Free Estriol competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Free Estriol in serum and plasma.

     

    A 96-well plate has been precoated with anti-Estriol IgG. Samples and the Estriol-HRP conjugate are added to the wells, where Estriol in the sample competes with the added Estriol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estriol in the sample and the intensity is measured at 450 nm.

  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.

Images

  • Representative Standard Curve using ab108671.

Protocols

References

ab108671 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us. We have a number of antibodies directed against estriol, ab64546, ab20278 and ab44962 are sheep, mouse and rat polyclonals respectively. These have all been tested with radioimmunoassay. Unfortunately we do not have conjugated estriol in our catalogue. However, we do have a kit, Estriol Human ELISA Kit (ab108671), which can be used to measure free (unconjugated) estriol in serum or plasma. I hope this information is helpful to you. If you have any additional questions please don't hesitate to ask.

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