Overview

  • Product name

    Human FSTL1 ELISA Kit
    See all FSTL1/FRP kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 1.34ng/ml 0.056 4.2%
    2 16 7.2ng/ml 0.382 5.3%
    3 16 13.9ng/ml 0.89 6.4%
    Inter-assay
    Sample n Mean SD CV%
    1 24 1.25ng/ml 0.094 7.5%
    2 24 7.5ng/ml 0.48 6.4%
    3 24 14.4ng/ml 1.123 7.8%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    312 pg/ml - 20000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human FSTL1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213782) is designed for the quantitative measurement of Human FSTL1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FSTL1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: M1-I308) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FSTL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FSTL1 amount of sample captured in plate.

  • Notes

    Follistatin-related protein 1, also called FSTL1 or FRP is a protein that in humans is encoded by the FSTL1 gene. FSTL1 gene is mapped to chromosome 3q13.33 based on an alignment of FSTL1 sequence with the genomic sequence (GRCh37). This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. This gene may modulate the action of some growth factors on cell proliferation and differentiation. It was suggested that fibroblast-secreted FSTL1 acts on macrophages to increase TNF and IL1B production and IL6 activity.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human FSTL1 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human FSTL1 Antibody 1 x 130µl
    Lyophilized Recombinant Human FSTL1 Standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin.
  • Tissue specificity

    Overexpressed in synovial tissues from rheumatoid arthritis.
  • Sequence similarities

    Contains 2 EF-hand domains.
    Contains 1 follistatin-like domain.
    Contains 1 Kazal-like domain.
    Contains 1 VWFC domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Anti FSTL1
    • FLJ50214
    • FLJ52277
    • Follistatin like 1
    • Follistatin related protein
    • Follistatin-like protein 1
    • Follistatin-related protein 1
    • FRP
    • FSL1
    • FSTL1
    • FSTL1 antibody
    • FSTL1_HUMAN
    • Micro RNA 198
    • MIR198
    • miRNA198 included
    • OCC 1
    • OCC1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human FSTL1 ELISA Kit (ab213782) Standard Curve.

Protocols

References

ab213782 has not yet been referenced specifically in any publications.

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