Human Furin ELISA Kit (ab282292)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.05 ng/ml
- Range: 0.16 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Furin ELISA Kit
See all Furin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 8 10.3% Inter-assay Sample n Mean SD CV% Plasma 3 13% -
Sample type
Cell culture supernatant, Saliva, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.05 ng/ml -
Range
0.16 ng/ml - 10 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 101 97% - 104% Serum 113 103% - 132% Hep Plasma 103 100% - 106% EDTA Plasma 99 98% - 101% Cit plasma 103 99% - 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Furin ELISA kit (ab282292) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Furin protein in human plasma, serum, saliva and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Furin with 0.05 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Furin, also referred to as dibasic-processing enzyme and Paired basic amino acid residue-cleaving enzyme (PACE), is a calcium-dependent serine endoprotease enzyme encoded by the FURIN gene. Furin belongs to the subtilisin-like proprotein convertase family S8. An 81 amino acid long inhibition peptide acts as a chaperone, is autocatalytically removed in the endoplasmic reticulum, and remains non-covalently bound until a second cleavage occurs in the trans Golgi. Furin acts on many substrates including but not limited to proparathroid hormone, transforming growth factor beta 1, proalbumin, and von Willebrand factor. Furin also acts on virus and is thought to activate the HIV envelope glycoproteins gp160 and gp140, and is thought to cleave the S1/S2 site of the spike protein of the SARS-CoV-2 coronavirus.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Furin Capture Antibody 1 x 600µl 10X Human Furin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI2 1 x 6ml Human Furin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. -
Tissue specificity
Seems to be expressed ubiquitously. -
Sequence similarities
Belongs to the peptidase S8 family. Furin subfamily.
Contains 1 homo B/P domain. -
Domain
Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface. -
Post-translational
modificationsThe inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin. - Information by UniProt
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Alternative names
- Dibasic processing enzyme
- Dibasic-processing enzyme
- FES upstream region
see all -
Database links
- Entrez Gene: 5045 Human
- Omim: 136950 Human
- SwissProt: P09958 Human
- Unigene: 513153 Human
Images
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The Furin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of Furin were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: 50% plasma (EDTA), 50% plasma (Citrate), 50% Plasma (Heparin), 25% serum, and 7.5% saliva. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 4.8 ng/mL in plasma (EDTA), 4.1 ng/mL in plasma (Citrate) and 4 ng/mL in and plasma (Heparin), 4.6 ng/mL in serum, and 76 ng/mL in saliva.
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The concentrations of Furin were measured in duplicate and interpolated from the Furin standard curves. Undiluted samples are PHA-M stimulated PBMC cell culture supernatant 50% and unstimulated PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Furin concentration was calculated to be 2.24 ng/mL in PHA-M stimulated PBMC cell culture supernatant and undetectable in the unstimulated PBMC control.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Furin concentration was determined to be 3.8 ng/mL with a range of 2.2 – 8.3 ng/mL.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab282292 has not yet been referenced specifically in any publications.