Key features and details
- Range: 0.1 ng/ml - 50 ng/ml
- Sample type: Cit plasma, EDTA Plasma
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Product nameHuman FX ELISA kit (total FX antigen)
See all Factor X kits
Intra-assay Sample n Mean SD CV% Sample 1 20 0.71ng/ml 0.039 5.44% Sample 2 20 2.97ng/ml 0.155 5.23% Sample 3 20 12.63ng/ml 0.095 2.45%
Sample typeEDTA Plasma, Cit plasma
Range0.1 ng/ml - 50 ng/ml
Sample specific recovery Sample type Average % Range Spike 98 % - %
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Human FX ELISA kit (total FX antigen) (ab272773) is intended for the quantitative determination of total Factor X antigen in human plasma.
Human Factor X will bind to the capture antibody coated on the microtiter plate. Factor X and Xa will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-human Factor X primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total Factor X present in the samples, is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human Factor X. Color development is proportional to the concentration of Factor X in the samples.
Factor X standard provided is calibrated against the WHO 4th International Standard.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-Human FX Primary Antibody Lyophilized Vial 1 vial Anti-Rabbit Streptavidin-HRP Secondary Reagent 1 vial FX ELISA Plate 1 unit Human FX Standard Lyophilized Vial 1 vial TMB Substrate 1 x 10ml
FunctionFactor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Tissue specificityPlasma; synthesized in the liver.
Involvement in diseaseDefects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.
modificationsThe vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
- Information by UniProt
- Activated factor Xa heavy chain
- Coagulation factor X
ab272773 has not yet been referenced specifically in any publications.