Overview

  • Product name

    Human Galectin 1 Matched Antibody Pair Kit
    See all Galectin 1 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    125 pg/ml - 8000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Galectin 1.


    Both capture and detector antibodies are rabbit monoclonal antibodies.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


    Buffer information:
    The capture antibody is glycerol free.
    The detector antibody contains glycerol.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Human Galectin 1 Capture Antibody 1 x 100µg 1 x 20µg
    Human Galectin 1 Detector Antibody 1 x 25µg 1 x 5µg
    Human Galectin 1 Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
  • Tissue specificity

    Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
  • Sequence similarities

    Contains 1 galectin domain.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names

    • 14 kDa laminin-binding protein
    • 14 kDa lectin
    • Beta galactoside binding lectin
    • Beta galactoside binding lectin L 14 I
    • beta galactoside binding protein
    • Beta-galactoside-binding lectin L-14-I
    • DKFZp686E23103
    • Gal 1
    • Gal-1
    • GAL1
    • Galaptin
    • Galbp
    • Galectin
    • Galectin-1
    • Galectin1
    • GBP
    • HBL
    • HLBP14
    • HPL
    • L 14.5
    • L-14.5
    • L14
    • Lactose binding lectin 1
    • Lactose-binding lectin 1
    • Lect14
    • Lectin galactoside binding soluble 1
    • Lectin galactoside-binding soluble 1
    • LEG1_HUMAN
    • LGALS 1
    • LGALS1
    • Lgals1 lectin galactose binding soluble 1
    • MAPK activating protein MP12
    • Putative MAPK activating protein MP12
    • Putative MAPK-activating protein PM12
    • S Lac lectin 1
    • S-Lac lectin 1
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab218170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab218170 has not yet been referenced specifically in any publications.

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