Since very few research groups work with porcine animal models, there are not many kits with specificity to pigs.Therefore, we usually try with human kits due to his huge similarity to human.
This is why we have tried to test our plasma samples from a atrial fibrillation porcine animal model. According to literature, Galectin3 is upregulated during atrial fibrillation and is released to the blood such a way it can be measured and detected in plasma samples. In our case, it was very interesting to quantify the levels of Galectin3 in plasma samples taken from the inner atrial cavity, since in case that Galectin3 is being released by the atrial cardiomyocytes, the atrial blood pool would have increased Galectin3 levels compared to peripheral blood.
However, in our experiment with your Human Galectin-3 ELISA Kit we have not been able to detect Gal3 levels in pig plasma samples. We have followed point by point the protocol of your kit, with the recommended dilutions of the Heparin plasma samples, making duplicates for statistical reasons and with several animal conditions to verify differences among different atrial fibrillation animal models.
In the attached image you can verify as the first 2 columns of the plate (A and B) correspond to the Standard Curve (first 2 wells -A1 and B1- correspond the blank wells and the rest of them are the decreasing concentrations points of the standard curve). The standard curve fits a 4PL curve with an r2=0.997. The rest of the wells are the pig plasma samples and all of them show a coloration deposit similar to the control blank control.
Measuring the OD450nm the results show that there is no any detection of Gal3 in our plasma samples from pigs.
Therefore, I would recommend this kit for using it with human samples. It is very easy, you can achieve your results in just one hour and the protocol is very easy to follow. However, it is clear that it is not a proper kit for pig samples since it is not able to detect any Galectin3 in this kind of samples.
Abcam user community
Submitted Dec 14 2020