Overview

  • Product name

    Human Galectin-3BP ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 477pg/ml 27.7 5.8%
    2 16 1541pg/ml 92.46 6%
    3 16 2626pg/ml 128.7 4.9%
    Inter-assay
    Sample n Mean SD CV%
    1 24 527pg/ml 32.15 6.1%
    2 24 1712pg/ml 114.7 6.7%
    3 24 3015pg/ml 168.84 5.6%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human Galectin-3BP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213784) is designed for the quantitative measurement of Human Galectin-3BP in cell culture supernatants, serum and plasma (heparin).


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-3BP has been pre-coated onto 96-well plates. Standards (NSO; V19-D585) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-3BP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Galectin-3BP amount of sample captured in plate.

  • Notes

    Galectin-3-binding protein is a protein that in humans is encoded by the LGALS3BP gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell–cell and cell–matrix interactions. Using fluorescence in situ hybridization, the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage associated lectin known as Mac-2 and also binds to galectin 1.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human Galectin-3BP coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Human Galectin-3BP antibody 1 x 130µl
    Lyophilized recombinant Human Galectin-3BP standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
  • Tissue specificity

    Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts.
  • Sequence similarities

    Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 1 SRCR domain.
  • Cellular localization

    Secreted. Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names

    • 90K
    • 90K MAC 2 BP
    • Basement membrane autoantigen p105
    • BTBD17B
    • Galectin 3 binding protein
    • Galectin-3-binding protein
    • L3 antigen
    • Lectin galactoside binding soluble 3 binding protein
    • Lectin galactoside-binding soluble 3-binding protein
    • LG3BP_HUMAN
    • LGALS3BP
    • M2BP
    • Mac 2 binding protein
    • Mac 2 BP
    • Mac-2 BP
    • Mac-2-binding protein
    • Mac2 BP
    • MAC2BP
    • Serum protein 90K
    • TANGO10B
    • Transport and golgi organization 10 homolog B
    • Tumor associated antigen 90K
    • Tumor-associated antigen 90K
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human Galectin-3BP ELISA Kit (ab213784) Standard Curve.

Protocols

References

ab213784 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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