Overview

  • Product name

    Human Galectin-4 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 0.48ng/ml 0.02 4.2%
    2 16 3.64ng/ml 0.14 3.8%
    3 16 6.25ng/ml 0.35 5.6%
    Inter-assay
    Sample n Mean SD CV%
    1 24 0.51ng/ml 0.03 5.9%
    2 24 4.02ng/ml 0.19 4.7%
    3 24 6.93ng/ml 0.43 6.2%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human Galectin-4 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213785) is designed for the quantitative measurement of Human Galectin-4 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). 


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-4 has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: A2-I323) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Galectin-4 amount of sample captured in plate.

  • Notes

    Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly under expressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab213785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human Galectin-4 ELISA Kit (ab213785) Standard Curve.

Protocols

References

ab213785 has not yet been referenced specifically in any publications.

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