Overview

  • Product name
    Human Galectin-9  / Gal-9 ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    1 16 526pg/ml 23.67 4.5%
    2 16 2335pg/ml 91.07 3.9%
    3 16 4518pg/ml 189.8 4.2%
    Inter-assay
    Sample n Mean SD CV%
    1 24 521pg/ml 38.03 7.3%
    2 24 2357pg/ml 143.8 6.1%
    3 24 4526pg/ml 294.2 6.5%
  • Sample type
    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    93.8 pg/ml - 6000 pg/ml
  • Assay time
    3h 30m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    The Human Galectin-9 / Gal-9 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213786) is designed for the quantitative detection of Human Galectin-9 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). 


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for Galectin-9 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: M1-T355) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Galectin-9 amount of sample captured in plate.

  • Notes

    Galectin-9, also called HUAT or LGALS9A is a protein that in humans is encoded by the LGALS9 gene. This gene is mapped to 17q11.2. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H and RS cells with their surrounding cells and might thus, play a role in the pathogenesis of this disease and/or its associated immunodeficiency. The protein has N- and C- terminal carbohydrate-binding domains connected by a link peptide.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human Galectin-9 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human Galectin-9 antibody 1 x 130µl
    Lyophilized recombinant Human Galectin-9 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas
  • Function
    Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant.
  • Tissue specificity
    Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue.
  • Sequence similarities
    Contains 2 galectin domains.
  • Domain
    Contains two homologous but distinct carbohydrate-binding domains.
  • Cellular localization
    Cytoplasm. Secreted. May also be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • Alternative names
    • 36 kDa beta-galactoside-binding lectin
    • Ecalectin
    • Gal-9
    • galectin 9
    • Galectin-9
    • galectin9
    • HOM HD 21
    • HOMHD21
    • HUAT
    • Lectin galactoside binding soluble 9
    • LEG9_HUMAN
    • LGAL S9
    • LGALS 9
    • Lgals9
    • LGALS9A
    • MGC117375
    • MGC125973
    • MGC125974
    • Tumor antigen HOM-HD-21
    • UAT
    • Urate transporter/channel
    • Urate transporter/channel protein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human Galectin-9 / Gal-9 ELISA Kit (ab213786) Standard Curve

Protocols

References

ab213786 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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