Overview

  • Product name

    Human GATA3 ELISA Kit
    See all GATA3 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    T47D Cells 9 3.2%
    Inter-assay
    Sample n Mean SD CV%
    T47D Cells 3 4.39%
  • Sample type

    Cell culture extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    7.29 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 107 106% - 108%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    GATA3 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of GATA3 protein in humancell extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    GATA3 is part of the GATA family of transcription factors. Trans-acting T-cell-specific transcription factor, or GATA3, is a zinc-binding transcriptional factor encoded by the GATA3 gene. GATA3 belongs to a group of transcription factors which are characterized by their ability to bind the DNA sequence “GATA”. The main function of GATA3 is to act as a main transcriptional factor for the differentiation of T helper 2 (Th2) cells. GATA3 also induces the differentiation of Th0 cells towards the Th2 subtype while suppressing differentiation to Th1 cells. GATA3 promotes the secretion of IL-4, IL-5, and IL-13 from Th2 cells.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPR 1 x 6ml
    10X Human GATA3 Capture Antibody 1 x 600µl
    10X Human GATA3 Detector Antibody 1 x 600µl
    Human GATA3 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
  • Tissue specificity

    T-cells and endothelial cells.
  • Involvement in disease

    Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
  • Sequence similarities

    Contains 2 GATA-type zinc fingers.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Alternative names

    • GATA 3
    • GATA binding factor 3
    • GATA binding protein 3
    • GATA-binding factor 3
    • Gata3
    • GATA3_HUMAN
    • HDR
    • HDRS
    • MGC2346
    • MGC5199
    • MGC5445
    • Trans acting T cell specific transcription factor GATA 3
    • Trans-acting T-cell-specific transcription factor GATA-3
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab230939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The concentrations of GATA3 were measured in duplicates, interpolated from the GATA3 standard curves and corrected for sample dilution. Undiluted samples are as follows: T47D cell extract 50 µg/mL and MCF7 cell extract 187.5 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GATA3 concentration in the undiluted samples was determined to be 1,621 µg/mL in T47D cell extract and 3,807 µg/mL in MCF7 cell extract.

  • The GATA3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Protocols

References

ab230939 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab230939.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up