Overview

  • Product name

    Human GCP-2 ELISA Kit (CXCL6)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 45 pg/ml
  • Range

    39.06 pg/ml - 2500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum = 117.3 107% - 127%
    Plasma = 120.5 104% - 137%
    Cell culture media = 112.6 96% - 133%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s GCP-2 (FGCP) Human (Enzyme-Linked Immunosorbent Assay) ELISA kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human GCP-2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human GCP-2 (FGCP) coated on a 96-well plate. Standards and samples are pipetted into the wells and GCP-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GCP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GCP-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab155431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155431

Protocols

References

This product has been referenced in:

  • Salyer ACD & David SA Transcriptomal signatures of vaccine adjuvants and accessory immunostimulation of sentinel cells by toll-like receptor 2/6 agonists. Hum Vaccin Immunother 14:1686-1696 (2018). Read more (PubMed: 29852079) »
See 1 Publication for this product

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